Thyroperoxidase Antibody (MoAb47): sc-58432

Thyroperoxidase Antibody (MoAb47) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thyroperoxidase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Thyroperoxidase antibody (MoAb47) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thyroperoxidase (TPO) is a crucial enzyme in the synthesis of thyroid hormones, facilitating the iodination of tyrosine residues in thyroglobulin, which is essential for the production of thyroxine (T4) and triiodothyronine (T3). This process is vital for regulating metabolism, growth, and development in vertebrates. TPO is a 933 amino acid type I transmembrane glycoprotein that not only plays a significant role in hormone synthesis but is also a major autoantigen implicated in autoimmune thyroid diseases, such as Hashimoto′s thyroiditis and Graves′ disease. TPO′s structure includes a large N-terminal myeloperoxidase-like domain, a complement control protein domain, and an epidermal growth factor-like domain, which are important for enzymatic activity and interactions with other proteins. Additionally, TPO mediates the organification and intracellular retention of radioiodide, which can lead to targeted tumor cell death, highlighting potential therapeutic implications. Mutations in the TPO gene can result in goitrous congenital hypothyroidism, a condition characterized by impaired iodide organification, underscoring TPO′s importance in thyroid function and overall endocrine health.

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Thyroperoxidase Antibody (MoAb47) References:

1. Degradation of human thyroperoxidase in the endoplasmic reticulum involves two different pathways depending on the folding state of the protein.  |  Fayadat, L., et al. 2000. J Biol Chem. 275: 15948-54. PMID: 10748076
2. Ectopic expression of the thyroperoxidase gene augments radioiodide uptake and retention mediated by the sodium iodide symporter in non-small cell lung cancer.  |  Huang, M., et al. 2001. Cancer Gene Ther. 8: 612-8. PMID: 11571539
3. Increasing diversity of human thyroperoxidase generated by alternative splicing. Characterized by molecular cloning of new transcripts with single- and multispliced mRNAs.  |  Ferrand, M., et al. 2003. J Biol Chem. 278: 3793-800. PMID: 12454013
4. Complement activation by direct C4 binding to thyroperoxidase in Hashimoto's thyroiditis.  |  Blanchin, S., et al. 2003. Endocrinology. 144: 5422-9. PMID: 12960013
5. Intracellular trafficking of thyroid peroxidase to the cell surface.  |  Kuliawat, R., et al. 2005. J Biol Chem. 280: 27713-8. PMID: 15917231
6. [Chronic urticaria with alterations of the thyroid function and thyroid peroxidase antibodies].  |  Fernandez Romero, DS. and Malbran, A. 2005. Medicina (B Aires). 65: 231-4. PMID: 16042134
7. Relationship between thyroid peroxidase T cell epitope restriction and antibody recognition of the autoantibody immunodominant region in human leukocyte antigen DR3 transgenic mice.  |  Guo, J., et al. 2005. Endocrinology. 146: 4961-7. PMID: 16081633
8. Estrogen effects on thyroid iodide uptake and thyroperoxidase activity in normal and ovariectomized rats.  |  Lima, LP., et al. 2006. Steroids. 71: 653-9. PMID: 16762383
9. Thyroperoxidase, thyroglobulin, Na(+)/I(-) symporter, pendrin in thyroid autoimmunity.  |  Czarnocka, B. 2011. Front Biosci (Landmark Ed). 16: 783-802. PMID: 21196203
10. Effect of levothyroxine on the pregnancy outcomes in recurrent pregnancy loss women with subclinical hypothyroidism and thyroperoxidase antibody positivity: a systematic review and meta-analysis.  |  Yu, M., et al. 2023. J Matern Fetal Neonatal Med. 36: 2233039. PMID: 37433649
11. Significance of thyroperoxidase and thyroglobulin antibodies in medically treated Graves' disease.  |  Constantinescu, SM., et al. 2023. Eur Thyroid J. 12: PMID: 37930957
12. Human thyroperoxidase is largely retained and rapidly degraded in the endoplasmic reticulum. Its N-glycans are required for folding and intracellular trafficking.  |  Fayadat, L., et al. 1998. Endocrinology. 139: 4277-85. PMID: 9751510

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thyroperoxidase Antibody (MoAb47) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-Thyroperoxidase Antibody (MoAb47) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thyroperoxidase Antibody (MoAb47) Specifications

Alternate Product Name: Thyroperoxidase

Product Clone Name: MoAb47

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Thyroperoxidase

Epitope Species: human

Product Reactivity/Detection: Thyroperoxidase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for Thyroperoxidase Antibody (MoAb47): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Thyroperoxidase antibody is Thyroperoxidase Antibody (A-5): sc-376876.