ThrRS Antibody (A-3): sc-166147

ThrRS Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects threonyl-tRNA synthetase (ThrRS) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ThrRS plays a crucial role in protein synthesis by catalyzing the aminoacylation of tRNA with threonine, thereby ensuring accurate translation of genetic information into functional proteins. This process is vital for cellular function and growth, as ThrRS directly impacts the fidelity of protein synthesis. ThrRS (A-3) antibody targets a protein localized in the cytoplasm, characterized by a complex structure including a zinc-binding catalytic domain, a C-terminal tRNA-binding domain, and an N-terminal editing domain. The intricate arrangement of these domains allows ThrRS to undergo conformational changes during catalysis, facilitating the binding of threonine to corresponding tRNA. Notably, ThrRS features four mobile regions, three of which contain key residues that alter their conformation throughout the catalytic cycle, enhancing the efficiency of the tRNA-amino acid reaction. The fourth mobile region includes an ordering loop that plays a critical role in closing the active site once threonine is positioned correctly, underscoring the importance of structural dynamics in maintaining accurate protein synthesis. Anti-ThrRS antibody (A-3) serves as an essential tool for researchers studying translation mechanisms and protein synthesis regulation in various biological contexts.

The preferred ThrRS antibody is ThrRS Antibody (C-3): sc-166146.