Anti-ThrRS Antibody (A-3) recommended for detection of ThrRS of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-ThrRS Antibody (A-3): sc-166147

Anti-ThrRS Antibody (A-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • ThrRS Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-98 mapping at the N-terminus of ThrRS of human origin
    • recommended for detection of ThrRS of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ThrRS Antibody (A-3) for WB applications. These reagents are now offered in bundles with ThrRS Antibody (A-3) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with ThrRS (A-3): sc-166147 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Produced positive Western Blot data of ThrRS Produced positive Western Blot data of ThrRS expression in non-transfected 293T, human ThrRS transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2013-05-21
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