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Thrombospondin 1 Antibody (A4.1): sc-59886

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Datasheets
  • Thrombospondin 1 Antibody (A4.1) is a mouse monoclonal IgM κ, cited in 22 publications, provided at 200 µg/ml
  • raised against calcium-replete native purified Thrombospondin of human origin
  • recommended for detection of Thrombospondin 1 of mouse, rat, human and bovine origin by WB, IP, IF and FCM; non cross-reactive with Fibronectin, fibrinogen or Von Willebrand factor
  • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • See Thrombospondin 1 (C-8): sc-393504 for Thrombospondin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Thrombospondin 1 Antibody (A4.1). This work is in progress.

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Thrombospondin 1 Antibody (A4.1) is a mouse monoclonal IgM kappa light chain antibody designed to target Thrombospondin 1 (TSP-1). TSP-1 is a key extracellular glycoprotein involved in cell-to-cell and cell-to-matrix signaling, playing a vital role in processes such as platelet aggregation, cell adhesion, migration, and the regulation of tissue development, repair, and angiogenesis. TSP-1 also plays a role in inhibiting angiogenesis in certain contexts and modulating the activity of growth factors like TGF-β. Anti-Thrombospondin 1 antibody (A4.1) is raised against calcium-replete native purified human Thrombospondin 1 and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM) applications. Thrombospondin 1 (A4.1) antibody demonstrates broad species reactivity, recognizing Thrombospondin 1 from mouse, rat, human, and bovine origins. The ability of TSP-1 to influence angiogenesis, wound healing, and cancer metastasis underscores its importance in both physiological and pathological contexts. Anti-Thrombospondin 1 antibody (A4.1) enables researchers to accurately investigate the diverse functions of TSP-1, facilitating advancements in understanding cellular mechanisms and developing therapeutic strategies. Thrombospondin 1 (A4.1) monoclonal antibody is an essential tool for studies focused on the intricate roles of TSP-1 in maintaining cellular homeostasis and responding to tissue injury.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Thrombospondin 1 Antibody (A4.1)

sc-59886
200 µg/ml
$316.00

Thrombospondin 1 Antibody (A4.1) FITC

sc-59886 FITC
200 µg/ml
$330.00

Thrombospondin 1 Antibody (A4.1) PE

sc-59886 PE
200 µg/ml
$343.00

Can Thrombospondin 1 (A4.1): sc-59886 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Thrombospondin 1 (A4.1): sc-59886 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_11, d_23, h_8CST
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Rated 4 out of 5 by from Worked nice for ICC with rat kidney samplesWorked nice for ICC with rat kidney samples. -SCBT Publication Review
Date published: 2015-03-04
Rated 4 out of 5 by from Worked well for Western with mouse samplesWorked well for Western with mouse samples. -SCBT Publication Review
Date published: 2015-01-16
Rated 4 out of 5 by from WB: Single clean band in CCDWB: Single clean band in CCD-1064Sk whole cell lysate. -SCBT QC
Date published: 2014-04-24
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Thrombospondin 1 Antibody (A4.1) is rated 4.0 out of 5 by 3.
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