THOC1 Antibody (E-10): sc-514123

THOC1 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects THOC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-THOC1 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. THOC1, also known as THO complex subunit 1, Tho1, P84, HPR1, or P84N5, is a 657 amino acid nuclear matrix protein that plays a crucial role in the regulation of gene expression and RNA processing. THOC1 (E-10) monoclonal antibody serves as a key component of the THO/TREX (transcription/export) complex, which is essential for coupling transcription with RNA splicing and export. This complex is recruited to actively transcribed genes and moves alongside RNA polymerase II during elongation, ensuring that mRNA is properly processed and transported out of the nucleus. THOC1 shows high expression levels in breast cancer cells compared to normal epithelial tissues; a reduction in THOC1 expression has been linked to decreased cell proliferation in cancer cell lines, indicating that cancer cells may rely on elevated THOC1 levels for growth and survival. Consequently, THOC1 presents a promising target for therapeutic intervention in cancer treatment, making anti-THOC1 antibody (E-10) an invaluable tool for researchers studying its role in cancer biology and gene regulation.

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THOC1 Antibody (E-10) References:

1. TREX is a conserved complex coupling transcription with messenger RNA export.  |  Strässer, K., et al. 2002. Nature. 417: 304-8. PMID: 11979277
2. Human hHpr1/p84/Thoc1 regulates transcriptional elongation and physically links RNA polymerase II and RNA processing factors.  |  Li, Y., et al. 2005. Mol Cell Biol. 25: 4023-33. PMID: 15870275
3. Combination gene therapy with p53 and Thoc1/p84 is more effective than either single agent in an animal model of human pancreatic adenocarcinoma.  |  Yin, S. and Goodrich, DW. 2006. Int J Oncol. 28: 781-5. PMID: 16465385
4. Thoc1/Hpr1/p84 is essential for early embryonic development in the mouse.  |  Wang, X., et al. 2006. Mol Cell Biol. 26: 4362-7. PMID: 16705185
5. FMIP controls the adipocyte lineage commitment of C2C12 cells by downmodulation of C/EBP alpha.  |  Mancini, A., et al. 2007. Oncogene. 26: 1020-7. PMID: 16909111
6. An allelic series for studying the mouse Thoc1 gene.  |  Wang, X., et al. 2007. Genesis. 45: 32-7. PMID: 17211872
7. Cancer cells and normal cells differ in their requirements for Thoc1.  |  Li, Y., et al. 2007. Cancer Res. 67: 6657-64. PMID: 17638875
8. The Thoc1 ribonucleoprotein and prostate cancer progression.  |  Chinnam, M., et al. 2014. J Natl Cancer Inst. 106: PMID: 25296641
9. Elevated expression of Thoc1 is associated with aggressive phenotype and poor prognosis in colorectal cancer.  |  Liu, C., et al. Biochem Biophys Res Commun. 468: 53-8. PMID: 26545775
10. THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis.  |  Zhang, L., et al. 2020. PLoS Genet. 16: e1008953. PMID: 32776944
11. THOC1 complexes with SIN3A to regulate R-loops and promote glioblastoma progression.  |  Budhiraja, S., et al. 2024. bioRxiv.. PMID: 39386597
12. The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing.  |  Durfee, T., et al. 1994. J Cell Biol. 127: 609-22. PMID: 7525595

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-THOC1 Antibody (E-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-THOC1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

THOC1 Antibody (E-10) Specifications

Alternate Product Name: THOC1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: THOC1

Epitope Species: human

Product Reactivity/Detection: THOC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for THOC1 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent