TH-POK Antibody (A-4): sc-376250

TH-POK Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TH-POK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TH-POK (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TH-POK, also known as T-helper-inducing POZ/Krüppel-like factor, zinc finger protein 67 (ZFP67), or Krüppel-related zinc finger protein cKrox, is a 539 amino acid protein characterized by one BTB (POZ) domain and four C2H2-type zinc fingers. TH-POK is primarily localized in the nucleus, where TH-POK plays a crucial role in regulating the lineage commitment of immature T-cell precursors. TH-POK directs positively selected thymocytes toward the CD4 lineage; without TH-POK, these cells default to the CD8 lineage, which is vital for maintaining the balance of T-cell populations in the immune system. TH-POK also acts as a transcriptional repressor for several genes, including COL1A1, COL1A2, and fibronectin, further underscoring TH-POK′s significance in T-cell development and function.

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TH-POK Antibody (A-4) References:

1. The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment.  |  He, X., et al. 2005. Nature. 433: 826-33. PMID: 15729333
2. Role of the transcription factor Th-POK in CD4:CD8 lineage commitment.  |  Kappes, DJ., et al. 2006. Immunol Rev. 209: 237-52. PMID: 16448546
3. CD4/CD8 lineage commitment: light at the end of the tunnel?  |  He, X. and Kappes, DJ. 2006. Curr Opin Immunol. 18: 135-42. PMID: 16480861
4. Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells.  |  Kimura, H., et al. 2006. Leukemia. 20: 1551-6. PMID: 16926850
5. Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development.  |  Setoguchi, R., et al. 2008. Science. 319: 822-5. PMID: 18258917
6. CD4-CD8 lineage commitment is regulated by a silencer element at the ThPOK transcription-factor locus.  |  He, X., et al. 2008. Immunity. 28: 346-58. PMID: 18342007
7. Distinct functions for the transcription factors GATA-3 and ThPOK during intrathymic differentiation of CD4(+) T cells.  |  Wang, L., et al. 2008. Nat Immunol. 9: 1122-30. PMID: 18776904
8. ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage.  |  Egawa, T. and Littman, DR. 2008. Nat Immunol. 9: 1131-9. PMID: 18776905
9. Putting ThPOK in place.  |  Bell, JJ. and Bhandoola, A. 2008. Nat Immunol. 9: 1095-7. PMID: 18800158
10. The transcription factor Th-POK negatively regulates Th17 differentiation in Vα14i NKT cells.  |  Engel, I., et al. 2012. Blood. 120: 4524-32. PMID: 23034280
11. MicroRNA-133b Regulation of Th-POK Expression and Dendritic Cell Signals Affect NKT17 Cell Differentiation in the Thymus.  |  Di Pietro, C., et al. 2016. J Immunol. 197: 3271-3280. PMID: 27605013
12. Th-POK regulates mammary gland lactation through mTOR-SREBP pathway.  |  Zhang, R., et al. 2018. PLoS Genet. 14: e1007211. PMID: 29420538

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TH-POK Antibody (A-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-TH-POK Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TH-POK Antibody (A-4) Specifications

Alternate Product Name: TH-POK

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 252-358

Epitope Target: TH-POK

Epitope Species: human

Product Reactivity/Detection: TH-POK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for TH-POK Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent