TGN38 Antibody (B-6): sc-166594

TGN38 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TGN38 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TGN38 Antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TGN38, also known as trans-Golgi network protein 2, is a type I integral membrane protein that plays a crucial role in sorting and transporting proteins within the cell. TGN38 is primarily located in the trans-Golgi network, where TGN38 facilitates the partitioning of nascent proteins into carrier vesicles destined for appropriate cellular locations. This localization is vital for maintaining cellular homeostasis and ensuring proteins reach their functional sites, thereby influencing various cellular processes such as secretion and membrane trafficking. The cytosolic domain of TGN38 interacts with AP2 Clathrin adaptor complexes through a specific tyrosine-containing motif, which is essential for internalization from the plasma membrane. Additionally, TGN38 undergoes post-translational modifications, including N- and O-linked glycosylation, which are important for stability and function, particularly in tissues such as the brain, lung, and kidney.

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TGN38 Antibody (B-6) References:

1. Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin.  |  Stephens, DJ. and Banting, G. 1999. J Biol Chem. 274: 30080-6. PMID: 10514494
2. Characterisation of the lumenal domain of TGN38 and effects of elevated expression of TGN38 on glycoprotein secretion.  |  Lee, SS. and Banting, G. 2002. Eur J Cell Biol. 81: 609-21. PMID: 12494998
3. Clathrin adaptor epsinR is required for retrograde sorting on early endosomal membranes.  |  Saint-Pol, A., et al. 2004. Dev Cell. 6: 525-38. PMID: 15068792
4. Retention and stimulus-dependent recycling of dense core vesicle content in neuroendocrine cells.  |  Bauer, RA., et al. 2004. J Cell Sci. 117: 2193-202. PMID: 15126621
5. Identification, molecular characterization and immunolocalization of an isoform of the trans-Golgi-network (TGN)-specific integral membrane protein TGN38.  |  Reaves, B., et al. 1992. Biochem J. 283 (Pt 2): 313-6. PMID: 1575675
6. Identification of different itineraries and retromer components for endosome-to-Golgi transport of TGN38 and Shiga toxin.  |  Lieu, ZZ. and Gleeson, PA. 2010. Eur J Cell Biol. 89: 379-93. PMID: 20138391
7. Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).  |  Luzio, JP., et al. 1990. Biochem J. 270: 97-102. PMID: 2204342
8. Strain-specific presence of two TGN38 isoforms and absence of TGN41 in mouse.  |  Kasai, K., et al. 1995. J Biol Chem. 270: 14471-6. PMID: 7540170
9. The SXYQRL sequence in the cytoplasmic domain of TGN38 plays a major role in trans-Golgi network localization.  |  Wong, SH. and Hong, W. 1993. J Biol Chem. 268: 22853-62. PMID: 8226795
10. An endocytosed TGN38 chimeric protein is delivered to the TGN after trafficking through the endocytic recycling compartment in CHO cells.  |  Ghosh, RN., et al. 1998. J Cell Biol. 142: 923-36. PMID: 9722606
11. Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120.  |  Stephens, DJ. and Banting, G. 1998. Biochem J. 335 (Pt 3): 567-72. PMID: 9794796
12. TGN38 cycles via the basolateral membrane of polarized Caco-2 cells.  |  Reaves, BJ., et al. 1998. Mol Membr Biol. 15: 133-9. PMID: 9859110

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TGN38 Antibody (B-6) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-TGN38 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TGN38 Antibody (B-6) Specifications

Alternate Product Name: TGOLN2

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-320

Epitope Target: TGN38

Epitope Species: human

Product Reactivity/Detection: precursor and mature TGN38 and isoforms TGN46, 48 and 51

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for TGN38 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin