TGN38 Antibody (A-5): sc-166224

TGN38 Antibody (A-5) is a mouse monoclonal IgG1 antibody that detects TGN38 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TGN38, also known as trans-Golgi network protein 2, is a type I integral membrane protein that plays a crucial role in sorting and transporting proteins within cells. TGN38 is primarily located in the trans-Golgi network and plasma membrane, where TGN38 facilitates the partitioning of nascent proteins into carrier vesicles destined for various cellular locations. This localization maintains cellular homeostasis and ensures proteins reach appropriate destinations, essential for proper cellular function. The cytosolic domain of TGN38 interacts with AP2 Clathrin adaptor complexes through a specific tyrosine-containing motif, enabling protein internalization from the plasma membrane. Additionally, TGN38 undergoes post-translational modifications, including N- and O-linked glycosylation, important for stability and function. These modifications contribute to TGN38′s presence in key organs such as the brain, lung, and kidney, highlighting TGN38′s significance in various physiological processes. Anti-TGN38 antibody (A-5) is an invaluable tool for researchers studying protein transport mechanisms and the role of the trans-Golgi network in cellular dynamics.

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TGN38 Antibody (A-5) References:

1. Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin.  |  Stephens, DJ. and Banting, G. 1999. J Biol Chem. 274: 30080-6. PMID: 10514494
2. The luminal domain of TGN38 interacts with integrin beta 1 and is involved in its trafficking.  |  Wang, J. and Howell, KE. 2000. Traffic. 1: 713-23. PMID: 11208159
3. Characterisation of the lumenal domain of TGN38 and effects of elevated expression of TGN38 on glycoprotein secretion.  |  Lee, SS. and Banting, G. 2002. Eur J Cell Biol. 81: 609-21. PMID: 12494998
4. Rapid dendritic transport of TGN38, a putative cargo receptor.  |  McNamara, JO., et al. 2004. Brain Res Mol Brain Res. 127: 68-78. PMID: 15306122
5. TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation.  |  Chen, L., et al. 2014. Cell Cycle. 13: 2723-32. PMID: 25486359
6. RGS4 controls Gαi3-mediated regulation of Bcl-2 phosphorylation on TGN38-containing intracellular membranes.  |  Bastin, G., et al. 2020. J Cell Sci. 133: PMID: 32501280
7. TGN38 recycles basolaterally in polarized Madin-Darby canine kidney cells.  |  Rajasekaran, AK., et al. 1994. Mol Biol Cell. 5: 1093-103. PMID: 7865877
8. The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network.  |  Ponnambalam, S., et al. 1994. J Cell Biol. 125: 253-68. PMID: 8163544
9. TGN38-green fluorescent protein hybrid proteins expressed in stably transfected eukaryotic cells provide a tool for the real-time, in vivo study of membrane traffic pathways and suggest a possible role for ratTGN38.  |  Girotti, M. and Banting, G. 1996. J Cell Sci. 109 (Pt 12): 2915-26. PMID: 9013339
10. Efficient trafficking of TGN38 from the endosome to the trans-Golgi network requires a free hydroxyl group at position 331 in the cytosolic domain.  |  Roquemore, EP. and Banting, G. 1998. Mol Biol Cell. 9: 2125-44. PMID: 9693371

The preferred TGN38 antibody is TGN38 Antibody (B-6): sc-166594.