TGIF Antibody (H-1): sc-17800

TGIF Antibody (H-1) is a mouse monoclonal IgG1 antibody that detects TGIF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TGIF (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TGIF protein, also known as TGFB Induced Factor Homeobox 1, plays a crucial role in regulating gene expression by acting as a transcriptional repressor. TGIF is primarily located in the nucleus, where TGIF interacts with other proteins, such as Smad2 and Smad4, to modulate TGFβ signaling pathways. This nuclear localization allows TGIF to directly influence transcriptional activity of genes involved in critical cellular processes, including development, differentiation, and response to extracellular signals. Through binding to the Smad2-Smad4 complex, TGIF can either repress or promote transcription depending on the cellular context, highlighting TGIF′s importance in maintaining cellular homeostasis and regulating responses to growth factors. TGIF′s ability to modulate these pathways underscores TGIF′s potential as a target for therapeutic interventions in diseases where TGFβ signaling is dysregulated.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TGIF Antibody (H-1) References:

1. A Smad transcriptional corepressor.  |  Wotton, D., et al. 1999. Cell. 97: 29-39. PMID: 10199400
2. Mutations in TGIF cause holoprosencephaly and link NODAL signalling to human neural axis determination.  |  Gripp, KW., et al. 2000. Nat Genet. 25: 205-8. PMID: 10835638
3. Homeobox gene TGIF-1 is increased in placental endothelial cells of human fetal growth restriction.  |  Gunatillake, T., et al. 2016. Reproduction. 152: 457-65. PMID: 27539603
4. Silencing of TGIF sensitizes MDA-MB-231 human breast cancer cells to cisplatin-induced apoptosis.  |  Pan, T., et al. 2018. Exp Ther Med. 15: 2978-2984. PMID: 29456703
5. TGFB-induced factor homeobox 1 (TGIF) expression in breast cancer.  |  Stürken, C., et al. 2021. BMC Cancer. 21: 920. PMID: 34391399
6. CtBP2 interacts with TGIF to promote the progression of esophageal squamous cell cancer through the Wnt/β‑catenin pathway.  |  Ju, Q., et al. 2022. Oncol Rep. 47: PMID: 34878149
7. A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif.  |  Bertolino, E., et al. 1995. J Biol Chem. 270: 31178-88. PMID: 8537382
8. A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway.  |  Baker, JC. and Harland, RM. 1996. Genes Dev. 10: 1880-9. PMID: 8756346
9. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways.  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
10. Analysis of TALE superclass homeobox genes (MEIS, PBC, KNOX, Iroquois, TGIF) reveals a novel domain conserved between plants and animals.  |  Bürglin, TR. 1997. Nucleic Acids Res. 25: 4173-80. PMID: 9336443
11. Physical and functional interaction of SMADs and p300/CBP.  |  Pouponnot, C., et al. 1998. J Biol Chem. 273: 22865-8. PMID: 9722503
12. TGF-beta-stimulated cooperation of smad proteins with the coactivators CBP/p300.  |  Janknecht, R., et al. 1998. Genes Dev. 12: 2114-9. PMID: 9679056

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TGIF Antibody (H-1) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-TGIF Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TGIF Antibody (H-1) Specifications

Alternate Product Name: TGIF1

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 100-272

Epitope Target: TGIF

Epitope Species: human

Product Reactivity/Detection: TGIF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for TGIF Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent