TGase3 CRISPR/Cas9 KO Plasmid (h): sc-406800
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TGase3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TGase3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TGase3 Antibody (H3): sc-101366
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase3 CRISPR/Cas9 KO Plasmid (h) | sc-406800 | 20 µg | $397.00 |
Overview
TGM3 encodes transglutaminase 3 (TGase3), a calcium-dependent enzyme that catalyzes protein crosslinking through formation of ε-(γ-glutamyl)lysine isopeptide bonds. TGase3 is best known for its role in terminal differentiation programs, contributing to stabilization of the cytoskeleton and extracellular protein assemblies during epithelial maturation and barrier formation. Its activity intersects with pathways governing keratinocyte differentiation, wound repair, and remodeling of structural proteins, linking enzymatic crosslinking to tissue homeostasis. Dysregulated transglutaminase activity and altered TGM3 expression have been associated with inflammatory and neoplastic processes in epithelial tissues, supporting investigation of TGase3 in disease-relevant differentiation states and tumor biology.
TGase3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TGM3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TGM3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TGM3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TGase3 protein expression.
This CRISPR knockout system enables efficient generation of TGM3-deficient cell models for investigation of TGase3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TGM3 exon(s) critical for TGase3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TGM3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TGase3 CRISPR/Cas9 KO Plasmid (h) and TGase3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TGM3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TGase3 HDR Plasmid (h) and TGase3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TGM3 homology arms to support homology-directed repair at defined TGM3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.