TGase3 Antibody (H3): sc-101366
- TGase3 Antibody (H3) is a mouse monoclonal IgG1 TGase3 antibody, cited in 1 publications, provided at 50 µg/0.5 ml
- raised against recombinant TGase3 of human origin
- recommended for detection of full length and proteolysed TGase3 of human origin by WB, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TGase3 Antibody (H3). This work is in progress.
QUICK LINKS
TGase3 Antibody (H3) is a mouse monoclonal IgG1 antibody that detects TGase3 protein of human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-TGase3 antibody (H3) is available as a non-conjugated format. TGase3, also known as transglutaminase-3, plays a crucial role in forming the cornified cell envelope, a vital structure that contributes to the integrity and barrier function of the epidermis. TGase3 is expressed during the late stages of terminal differentiation in the epidermis and in specific cell types within the hair follicle, highlighting TGase3′s importance in skin health and maintenance. TGase3 catalyzes the post-translational modification of proteins through the transamidation of glutamine residues, leading to the formation of stable, insoluble macromolecular structures essential for the epidermal barrier. TGase3 is a zymogen that requires proteolytic activation for enzymatic activity, and TGase3′s transglutaminase activity can be inhibited by guanosine triphosphate (GTP), similar to tissue-type transglutaminase (TGase2). TGase3 gene is located on human chromosome 20q11.2, and TGase3′s differential expression across various tissues underscores TGase3′s significance in both normal physiology and potential pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TGase3 Antibody (H3) References:
- Characterization of recombinant mouse epidermal-type transglutaminase (TGase 3): regulation of its activity by proteolysis and guanine nucleotides. | Hitomi, K., et al. 1999. J Biochem. 125: 1048-54. PMID: 10348906
- GTP, an inhibitor of transglutaminases, is hydrolyzed by tissue-type transglutaminase (TGase 2) but not by epidermal-type transglutaminase (TGase 3). | Hitomi, K., et al. 2000. Biosci Biotechnol Biochem. 64: 657-9. PMID: 10803976
- Analysis of epidermal-type transglutaminase (TGase 3) expression in mouse tissues and cell lines. | Hitomi, K., et al. 2001. Int J Biochem Cell Biol. 33: 491-8. PMID: 11331204
- Three-dimensional structure of the human transglutaminase 3 enzyme: binding of calcium ions changes structure for activation. | Ahvazi, B., et al. 2002. EMBO J. 21: 2055-67. PMID: 11980702
- Site-directed mutation in conserved anionic regions of guinea pig liver transglutaminase. | Ikura, K., et al. 1995. Arch Biochem Biophys. 318: 307-13. PMID: 7733658
- The proximal promoter of the human transglutaminase 3 gene. Stratified squamous epithelial-specific expression in cultured cells is mediated by binding of Sp1 and ets transcription factors to a proximal promoter element. | Lee, JH., et al. 1996. J Biol Chem. 271: 4561-8. PMID: 8626812
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase3 Antibody (H3) | sc-101366 | 50 µg/0.5 ml | $316.00 |