TFIIIC220 Antibody (F-12): sc-398780
- TFIIIC220 Antibody (F-12) is a mouse monoclonal IgG2a κ TFIIIC220 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of TFIIIC220 of human origin
- TFIIIC220 Antibody (F-12) is recommended for detection of TFIIIC220 of human origin by WB, IP, IF and ELISA
- Anti-TFIIIC220 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398780 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TFIIIC220 Antibody (F-12) for WB applications. These reagents are now offered in bundles with TFIIIC220 Antibody (F-12) (see ordering information below).
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TFIIIC220 Antibody (F-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects TFIIIC220 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIIC220 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIIC220 plays a crucial role in gene transcription by RNA polymerase III, which synthesizes essential RNA molecules such as tRNA and 5S rRNA. TFIIIC220 functions as a key component of the TFIIIC2 transcription factor complex, composed of several subunits including TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63. This complex recruits RNA polymerase III and the TFIIIB complex to promoter elements, facilitating transcription of genes necessary for cell survival and function. TFIIIC220 expression and associated subunits are often upregulated in malignant cells, such as those found in ovarian carcinomas, highlighting a potential role in cancer biology and transcriptional control deregulation in tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TFIIIC220 Antibody (F-12) References:
- The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity. | Hsieh, YJ., et al. 1999. Mol Cell Biol. 19: 7697-704. PMID: 10523658
- Interactions between the tetratricopeptide repeat-containing transcription factor TFIIIC131 and its ligand, TFIIIB70. Evidence for a conformational change in the complex. | Moir, RD., et al. 2000. J Biol Chem. 275: 26591-8. PMID: 10859316
- Alternatively spliced hBRF variants function at different RNA polymerase III promoters. | McCulloch, V., et al. 2000. EMBO J. 19: 4134-43. PMID: 10921893
- Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. | Schramm, L., et al. 2000. Genes Dev. 14: 2650-63. PMID: 11040218
- RNA polymerase III transcription factor TFIIIC2 is overexpressed in ovarian tumors. | Winter, AG., et al. 2000. Proc Natl Acad Sci U S A. 97: 12619-24. PMID: 11058163
- Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription. | Sutcliffe, JE., et al. 2000. Mol Cell Biol. 20: 9192-202. PMID: 11094071
- RNA polymerase III transcription: its control by tumor suppressors and its deregulation by transforming agents. | Brown, TR., et al. 2000. Gene Expr. 9: 15-28. PMID: 11097422
- Activation of mouse genes in transformed cells. | Scott, MR., et al. 1983. Cell. 34: 557-67. PMID: 6311432
- Expression of neural BC1 RNA: induction in murine tumours. | Chen, W., et al. 1997. Eur J Cancer. 33: 288-92. PMID: 9135502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIIC220 Antibody (F-12) | sc-398780 | 200 µg/ml | $322.00 | |||
TFIIIC220 Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-530926 | 200 µg Ab; 10 µg BP | $361.00 | |||
TFIIIC220 Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-524482 | 200 µg Ab, 40 µg BP | $361.00 | |||
TFIIIC220 Antibody (F-12): m-IgG2a BP-HRP Bundle | sc-547634 | 200 µg Ab; 10 µg BP | $361.00 | |||
TFIIIC220 Antibody (F-12) X | sc-398780 X | 200 µg/0.1 ml | $322.00 | |||
TFIIIC220 Antibody (F-12) AC | sc-398780 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TFIIIC220 Antibody (F-12) HRP | sc-398780 HRP | 200 µg/ml | $322.00 | |||
TFIIIC220 Antibody (F-12) FITC | sc-398780 FITC | 200 µg/ml | $336.00 | |||
TFIIIC220 Antibody (F-12) PE | sc-398780 PE | 200 µg/ml | $349.00 | |||
TFIIIC220 Antibody (F-12) Alexa Fluor® 488 | sc-398780 AF488 | 200 µg/ml | $364.00 | |||
TFIIIC220 Antibody (F-12) Alexa Fluor® 546 | sc-398780 AF546 | 200 µg/ml | $364.00 | |||
TFIIIC220 Antibody (F-12) Alexa Fluor® 594 | sc-398780 AF594 | 200 µg/ml | $364.00 | |||
TFIIIC220 Antibody (F-12) Alexa Fluor® 647 | sc-398780 AF647 | 200 µg/ml | $364.00 | |||
TFIIIC220 Antibody (F-12) Alexa Fluor® 680 | sc-398780 AF680 | 200 µg/ml | $364.00 | |||
TFIIIC220 Antibody (F-12) Alexa Fluor® 790 | sc-398780 AF790 | 200 µg/ml | $364.00 |