TFIIE-β Antibody (A-1): sc-137000
- TFIIE-β Antibody (A-1) is a mouse monoclonal IgG2b κ TFIIE-β antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-291 of TFIIE-β of human origin
- TFIIE-beta Antibody (A-1) is recommended for detection of TFIIE-β p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TFIIE-beta Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-137000 X, 200 µg/0.1 ml)
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TFIIE-β Antibody (A-1) for WB and IHC(P) applications. These reagents are now offered in bundles with TFIIE-β Antibody (A-1) (see ordering information below).
TFIIE-β Antibody (A-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects TFIIE-beta of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TFIIE-beta antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIE-beta plays a crucial role in transcription initiation, functioning as a key component of the transcription factor complex that interacts with RNA polymerase II. This interaction enables accurate and efficient transcription of protein-coding genes, as TFIIE-beta helps stabilize the transcription machinery at the promoter region. TFIIE-beta′s structure, consisting of two subunits forming a heterotetramer (α2β2), is essential for optimal transcription levels. By facilitating transcription complex assembly, TFIIE-beta ensures proper regulation of gene expression, making TFIIE-beta a critical factor in cellular function and development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TFIIE-β Antibody (A-1) References:
- TFIIA induces conformational changes in TFIID via interactions with the basic repeat. | Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
- Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau). | Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
- Structure and functional properties of human general transcription factor IIE. | Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
- Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE. | Ohkuma, Y., et al. 1991. Nature. 354: 398-401. PMID: 1956403
- Conserved sequence motifs in the small subunit of human general transcription factor TFIIE. | Sumimoto, H., et al. 1991. Nature. 354: 401-4. PMID: 1956404
- Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. | Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
- Functional domains and upstream activation properties of cloned human TATA binding protein. | Peterson, MG., et al. 1990. Science. 248: 1625-30. PMID: 2363050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIE-β Antibody (A-1) | sc-137000 | 200 µg/ml | $322.00 | |||
TFIIE-β Antibody (A-1): m-IgGκ BP-HRP Bundle | sc-521389 | 200 µg Ab, 40 µg BP | $361.00 | |||
TFIIE-β Antibody (A-1): m-IgG2b BP-HRP Bundle | sc-548888 | 200 µg Ab; 10 µg BP | $361.00 | |||
TFIIE-β Antibody (A-1) X | sc-137000 X | 200 µg/0.1 ml | $322.00 | |||
TFIIE-β Antibody (A-1) AC | sc-137000 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TFIIE-β Antibody (A-1) HRP | sc-137000 HRP | 200 µg/ml | $322.00 | |||
TFIIE-β Antibody (A-1) FITC | sc-137000 FITC | 200 µg/ml | $336.00 | |||
TFIIE-β Antibody (A-1) PE | sc-137000 PE | 200 µg/ml | $349.00 | |||
TFIIE-β Antibody (A-1) Alexa Fluor® 488 | sc-137000 AF488 | 200 µg/ml | $364.00 | |||
TFIIE-β Antibody (A-1) Alexa Fluor® 546 | sc-137000 AF546 | 200 µg/ml | $364.00 | |||
TFIIE-β Antibody (A-1) Alexa Fluor® 594 | sc-137000 AF594 | 200 µg/ml | $364.00 | |||
TFIIE-β Antibody (A-1) Alexa Fluor® 647 | sc-137000 AF647 | 200 µg/ml | $364.00 | |||
TFIIE-β Antibody (A-1) Alexa Fluor® 680 | sc-137000 AF680 | 200 µg/ml | $364.00 | |||
TFIIE-β Antibody (A-1) Alexa Fluor® 790 | sc-137000 AF790 | 200 µg/ml | $364.00 |