Date published: 2026-6-4

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TFIIE-α Antibody (F-2): sc-133065

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Datasheets
  • TFIIE-α Antibody (F-2) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 140-439 mapping at the C-terminus of TFIIE-α of human origin
  • recommended for detection of TFIIE-α of mouse, rat and human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-133065 X, 200 µg/0.1 ml)
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TFIIE-α Antibody (F-2). This work is in progress.
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TFIIE-α Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects TFIIE-α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIE-α plays a crucial role in the transcription initiation process by RNA polymerase II, functioning as a key component of the transcription factor complex that assembles at the promoter region of protein-coding genes. TFIIE-α is essential for the recruitment of RNA polymerase II and other transcription factors, facilitating the formation of the pre-initiation complex necessary for accurate transcription. TFIIE-α′s significance extends beyond its structural role, influencing the efficiency and regulation of gene expression, which is vital for cellular function and response to environmental signals. The TFIIE complex, consisting of TFIIE-α and its partner subunit TFIIE-β, is critical for the transition from transcription initiation to elongation, underscoring its significance in gene regulation. TFIIE-α′s ability to interact with other transcription factors and involvement in the dynamic process of transcription initiation make anti-TFIIE-α antibody (F-2) an indispensable tool for scientists studying the intricate mechanisms of transcription and its implications in various biological processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

TFIIE-α Antibody (F-2)

sc-133065
200 µg/ml
$322.00

TFIIE-α Antibody (F-2) X

sc-133065 X
200 µg/0.1 ml
$322.00

When should the TransCruz reagent sc-133065 X be used instead of the standard form sc-133065?

Asked by: Randy McDonald
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good specificity and signal strength in HeLa and AGood specificity and signal strength in HeLa and A-431 whole cell lysates and Jurkat, 3611-RF and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Published WB data with hamster InR1G9 cellsPublished WB data with hamster InR1G9 cells - SCBT Publication Review
Date published: 2014-07-22
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TFIIE-α Antibody (F-2) is rated 5.0 out of 5 by 2.
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