TFIIE-α Antibody (E-2): sc-133064
- TFIIE-α Antibody (E-2) is a mouse monoclonal IgG2a κ TFIIE-α antibody provided at 200 µg/ml
- raised against amino acids 140-439 mapping at the C-terminus of TFIIE-α of human origin
- TFIIE-alpha Antibody (E-2) is recommended for detection of TFIIE-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TFIIE-alpha Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-133064 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for TFIIE-α Antibody (E-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with TFIIE-α Antibody (E-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
TFIIE-α Antibody (E-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects TFIIE-alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TFIIE-alpha antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIE-alpha plays a crucial role in transcription initiation, functioning as a key component of the transcription factor complex that interacts with RNA polymerase II and other basal transcription factors. TFIIE-alpha is essential for recruiting RNA polymerase II to the promoter region of protein-coding genes, facilitating pre-initiation complex formation. This process ensures accurate gene expression, which regulates various cellular functions and responses. TFIIE complex consists of two subunits, α and β, forming a heterotetramer (α2β2) necessary for optimal transcription activity. TFIIE-alpha′s proper functioning is critical, as dysregulation can lead to aberrant gene expression, potentially contributing to various diseases, including cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TFIIE-α Antibody (E-2) References:
- TFIIA induces conformational changes in TFIID via interactions with the basic repeat. | Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
- Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau). | Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
- Structure and functional properties of human general transcription factor IIE. | Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
- Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE. | Ohkuma, Y., et al. 1991. Nature. 354: 398-401. PMID: 1956403
- Conserved sequence motifs in the small subunit of human general transcription factor TFIIE. | Sumimoto, H., et al. 1991. Nature. 354: 401-4. PMID: 1956404
- Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. | Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
- Functional domains and upstream activation properties of cloned human TATA binding protein. | Peterson, MG., et al. 1990. Science. 248: 1625-30. PMID: 2363050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIE-α Antibody (E-2) | sc-133064 | 200 µg/ml | $322.00 | |||
TFIIE-α Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-521221 | 200 µg Ab, 40 µg BP | $361.00 | |||
TFIIE-α Antibody (E-2) X | sc-133064 X | 200 µg/0.1 ml | $322.00 | |||
TFIIE-α Antibody (E-2) AC | sc-133064 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TFIIE-α Antibody (E-2) HRP | sc-133064 HRP | 200 µg/ml | $322.00 | |||
TFIIE-α Antibody (E-2) FITC | sc-133064 FITC | 200 µg/ml | $336.00 | |||
TFIIE-α Antibody (E-2) PE | sc-133064 PE | 200 µg/ml | $349.00 | |||
TFIIE-α Antibody (E-2) Alexa Fluor® 488 | sc-133064 AF488 | 200 µg/ml | $364.00 | |||
TFIIE-α Antibody (E-2) Alexa Fluor® 546 | sc-133064 AF546 | 200 µg/ml | $364.00 | |||
TFIIE-α Antibody (E-2) Alexa Fluor® 594 | sc-133064 AF594 | 200 µg/ml | $364.00 | |||
TFIIE-α Antibody (E-2) Alexa Fluor® 647 | sc-133064 AF647 | 200 µg/ml | $364.00 | |||
TFIIE-α Antibody (E-2) Alexa Fluor® 680 | sc-133064 AF680 | 200 µg/ml | $364.00 | |||
TFIIE-α Antibody (E-2) Alexa Fluor® 790 | sc-133064 AF790 | 200 µg/ml | $364.00 |