TFIIB Antibody (D-3): sc-271736

TFIIB Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIB (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIB protein plays a crucial role in the initiation of transcription by RNA polymerase II, acting as a key component of the transcription machinery. TFIIB is essential for the formation of the pre-initiation complex, which is necessary for accurate transcription of protein-coding genes. TFIIB interacts with the TATA-binding protein and RNA polymerase II, facilitating the recruitment of these components to the core promoter region. This interaction is vital for the proper assembly of the transcription machinery, ensuring that gene expression is regulated accurately. TFIIB′s ability to bridge the gap between the transcription factors and RNA polymerase II underscores TFIIB′s importance in the transcription process, making anti-TFIIB antibody (D-3) an invaluable tool for researchers studying gene regulation and transcriptional control in various biological contexts.

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TFIIB Antibody (D-3) References:

1. TFIIA induces conformational changes in TFIID via interactions with the basic repeat.  |  Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
2. Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau).  |  Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
3. Binding of general transcription factor TFIIB to an acidic activating region.  |  Lin, YS., et al. 1991. Nature. 353: 569-71. PMID: 1922364
4. Sequence of general transcription factor TFIIB and relationships to other initiation factors.  |  Malik, S., et al. 1991. Proc Natl Acad Sci U S A. 88: 9553-7. PMID: 1946368
5. Structure and functional properties of human general transcription factor IIE.  |  Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
6. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.  |  Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
7. Promoter independent abortive transcription assays unravel functional interactions between TFIIB and RNA polymerase.  |  Wiesler, SC., et al. 2013. Methods Mol Biol. 977: 217-27. PMID: 23436365
8. Functional domains and upstream activation properties of cloned human TATA binding protein.  |  Peterson, MG., et al. 1990. Science. 248: 1625-30. PMID: 2363050
9. Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.  |  O'Brien, MJ. and Ansari, A. 2024. Biochim Biophys Acta Proteins Proteom. 1872: 140968. PMID: 37863410
10. Domain structure of a human general transcription initiation factor, TFIIF.  |  Yonaha, M., et al. 1993. Nucleic Acids Res. 21: 273-9. PMID: 8441635
11. Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity.  |  Hisatake, K., et al. 1993. Nature. 363: 744-7. PMID: 8515820
12. Transcriptional activation by TFIIB mutants that are severely impaired in interaction with promoter DNA and acidic activation domains.  |  Chou, S. and Struhl, K. 1997. Mol Cell Biol. 17: 6794-802. PMID: 9372910

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIB Antibody (D-3) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIB Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIB Antibody (D-3) Specifications

Alternate Product Name: GTF2B

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 289-318

Epitope Target: TFIIB

Epitope Species: human

Product Reactivity/Detection: TFIIB p33

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for TFIIB Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent