TFIIB Antibody (D-12): sc-271784

TFIIB Antibody (D-12) is a mouse monoclonal IgM antibody that detects TFIIB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIB plays a crucial role in the transcription initiation process by serving as a bridge between the TATA-binding protein (TBP) and RNA polymerase II, facilitating the assembly of the transcription pre-initiation complex. This interaction is vital for the accurate transcription of protein-coding genes, as TFIIB ensures that RNA polymerase II is properly positioned at the promoter region, allowing for the precise regulation of gene expression. TFIIB′s importance extends beyond its structural role; TFIIB also influences the recruitment of additional transcription factors and co-activators, thereby modulating the transcriptional response to various cellular signals. Anti-TFIIB antibody (D-12) enables researchers to gain insights into the dynamics of transcription regulation and the intricate mechanisms that govern gene expression in eukaryotic cells.

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TFIIB Antibody (D-12) References:

1. TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism.  |  Hori, RT., et al. 2004. Nucleic Acids Res. 32: 3856-63. PMID: 15272087
2. A transcription-independent role for TFIIB in gene looping.  |  Singh, BN. and Hampsey, M. 2007. Mol Cell. 27: 806-16. PMID: 17803944
3. TFIIB aptamers inhibit transcription by perturbing PIC formation at distinct stages.  |  Sevilimedu, A., et al. 2008. Nucleic Acids Res. 36: 3118-27. PMID: 18403417
4. New insights into the role of TFIIB in transcription initiation.  |  Wang, Y. and Roberts, SG. 2010. Transcription. 1: 126-129. PMID: 21326885
5. TFIIB-related factor 2 is associated with poor prognosis of nonsmall cell lung cancer patients through promoting tumor epithelial-mesenchymal transition.  |  Tian, Y., et al. 2014. Biomed Res Int. 2014: 530786. PMID: 24738062
6. TFIIB is only ∼9 Å away from the 5'-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex.  |  Bick, MJ., et al. 2015. PLoS One. 10: e0119007. PMID: 25774659
7. Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.  |  Eychenne, T., et al. 2016. Genes Dev. 30: 2119-2132. PMID: 27688401
8. Release of Human TFIIB from Actively Transcribing Complexes Is Triggered upon Synthesis of 7- and 9-nt RNAs.  |  Ly, E., et al. 2020. J Mol Biol. 432: 4049-4060. PMID: 32417370
9. Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.  |  Yamashita, S., et al. 1993. Science. 261: 463-6. PMID: 8332911
10. Potential RNA polymerase II-induced interactions of transcription factor TFIIB.  |  Malik, S., et al. 1993. Mol Cell Biol. 13: 6253-9. PMID: 8413225

The preferred TFIIB antibody is TFIIB Antibody (D-3): sc-271736.