Anti-TFII-I Antibody (B-7) is a mouse monoclonal IgG1 κ TFII-I antibody, cited in 2 publications, provided at 200 µg/ml
raised against amino acids 941-998 of TFII-I of human origin
Anti-TFII-I Antibody (B-7) is recommended for detection of short and long isoforms of TFII-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-TFII-I Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-46670 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFII-I (B-7): sc-46670.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TFII-I Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with TFII-I Antibody (B-7) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
TFII-I Antibody (B-7) is a high quality monoclonal TFII-I antibody (also designated TFII-I antibody) suitable for the detection of the TFII-I protein of mouse, rat and human origin. TFII-I Antibody (B-7) is available as both the non-conjugated anti-TFII-I antibody form, as well as multiple conjugated forms of anti-TFII-I antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Initiation of transcription of eukaryotic genes requires the association of large multimeric protein complexes that involve RNA polymerase II and a variety of basal transcription factors, including members of the TFII protein family. TFII proteins complex with Pol II and initiate transcription by binding to the core promoter elements, such as TATA box sequences, that are located upstream of the transcription start codon. A member of the TFII family, TFII-I is regulated by tyrosine phophorylation, and it is involved in the initiation of transcription of TATA-less promoters and in cell type specific transcription. TFII-I directly associates with several promoters elements, including TATA box, pyrimidine-rich initiator (Inr) and E-box elements. TFII-I is also implicated in activating transcription of the transcription factor c-fos, a downstream effector protein in the MAP kinase-signaling pathway. TFII-I binds to several initiation sites within the c-fos promoter, and it is phosphorylated in response to Map kinase activation, which then enhances TFII-I induced expression of c-fos.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences