TFII-I Antibody (B-7): sc-46670

TFII-I Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFII-I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TFII-I (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFII-I protein plays a crucial role in regulating gene expression, particularly in TATA-less promoters, which are essential for transcribing many genes that lack a traditional TATA box. This characteristic is particularly important as TATA-less promoters are often found in genes expressed in a tissue-specific manner or in response to specific signaling pathways. TFII-I not only binds to various core promoter elements, such as pyrimidine-rich initiator and E-box elements, but also activates c-fos transcription factor, a key player in MAP kinase signaling pathway. Upon activation, TFII-I undergoes tyrosine phosphorylation, enhancing c-fos expression and linking extracellular signals to transcriptional responses. This multifaceted role underscores TFII-I′s significance in cellular processes, making anti-TFII-I antibody (B-7) an invaluable tool for researchers studying transcriptional regulation and signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TFII-I Antibody (B-7) References:

1. Extracellular signal-regulated kinase binds to TFII-I and regulates its activation of the c-fos promoter.  |  Kim, DW. and Cochran, BH. 2000. Mol Cell Biol. 20: 1140-8. PMID: 10648599
2. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.  |  Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
3. Pathophysiology of TFII-I: Old Guard Wearing New Hats.  |  Roy, AL. 2017. Trends Mol Med. 23: 501-511. PMID: 28461154
4. TFII-I/Gtf2i and Erythro-Megakaryopoiesis.  |  Gurumurthy, A., et al. 2020. Front Physiol. 11: 590180. PMID: 33101065
5. Regulation of RNA Polymerase II Transcription Initiation and Elongation by Transcription Factor TFII-I.  |  Linzer, N., et al. 2021. Front Mol Biosci. 8: 681550. PMID: 34055891
6. Thymic stroma and TFII-I: towards new targeted therapies.  |  Manti, PG., et al. 2022. Trends Mol Med. 28: 67-78. PMID: 34865984
7. An alternative pathway for transcription initiation involving TFII-I.  |  Roy, AL., et al. 1993. Nature. 365: 355-9. PMID: 8377828
8. TFII-I enhances activation of the c-fos promoter through interactions with upstream elements.  |  Kim, DW., et al. 1998. Mol Cell Biol. 18: 3310-20. PMID: 9584171
9. Recruitment of human TBP selectively activates RNA polymerase II TATA-dependent promoters.  |  Majello, B., et al. 1998. J Biol Chem. 273: 16509-16. PMID: 9632719
10. TFII-I regulates Vbeta promoter activity through an initiator element.  |  Cheriyath, V., et al. 1998. Mol Cell Biol. 18: 4444-54. PMID: 9671454
11. Regulation of TFII-I activity by phosphorylation.  |  Novina, CD., et al. 1998. J Biol Chem. 273: 33443-8. PMID: 9837922
12. Dissecting the regulatory circuitry of a eukaryotic genome.  |  Holstege, FC., et al. 1998. Cell. 95: 717-28. PMID: 9845373

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFII-I Antibody (B-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TFII-I Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFII-I Antibody (B-7) Specifications

Alternate Product Name: TFII-I

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 941-998

Epitope Target: TFII-I

Epitope Species: human

Product Reactivity/Detection: short and long isoforms of TFII-I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TFII-I Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent