Tec Antibody (G-5): sc-365533

Tec Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tec protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tec (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tec protein plays a crucial role in signaling pathways of hematopoietic cells, particularly in T and B cell activation, which is vital for immune response. Tec (G-5) monoclonal antibody recognizes a member of the Tec family of non-receptor tyrosine kinases, which includes five other proteins: Emt, Btk, Bmx, Txk, and Dsrc28C. Each of these proteins contains SH3 and SH2 domains, essential for protein-protein interactions, and most also possess a pleckstrin homology (PH) domain and a Tec homology (TH) domain that facilitate membrane localization and signaling. Proper functioning of Tec is critical, as mutations can lead to immune deficiencies, such as X-linked agammaglobulinemia associated with Btk. Expression of Tec and its family members is tightly regulated, with specific isoforms being expressed in various hematopoietic lineages and tissues, underscoring their importance in both normal immune function and potential pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Tec Antibody (G-5) References:

1. The role of Tec family kinases in the regulation of T-helper-cell differentiation.  |  Boucheron, N. and Ellmeier, W. 2012. Int Rev Immunol. 31: 133-54. PMID: 22449074
2. Adult thymic epithelial cell (TEC) progenitors and TEC stem cells: Models and mechanisms for TEC development and maintenance.  |  Hamazaki, Y. 2015. Eur J Immunol. 45: 2985-93. PMID: 26362014
3. Regulation of the Tec family of non-receptor tyrosine kinases in cardiovascular disease.  |  Yin, Z., et al. 2022. Cell Death Discov. 8: 119. PMID: 35296647
4. Improvements in immune/melanocyte biomarkers with JAK3/TEC family kinase inhibitor ritlecitinib in vitiligo.  |  Guttman-Yassky, E., et al. 2024. J Allergy Clin Immunol. 153: 161-172.e8. PMID: 37777018
5. Ritlecitinib, a JAK3/TEC family kinase inhibitor, stabilizes active lesions and repigments stable lesions in vitiligo.  |  Yamaguchi, Y., et al. 2024. Arch Dermatol Res. 316: 478. PMID: 39023568
6. A review of TEC family kinases and their inhibitors in the treatment of alopecia areata.  |  An, Y. and Zhang, F. 2024. Arch Dermatol Res. 316: 496. PMID: 39080140
7. CD28 is associated with and induces the immediate tyrosine phosphorylation and activation of the Tec family kinase ITK/EMT in the human Jurkat leukemic T-cell line.  |  August, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 9347-51. PMID: 7524075
8. Identification of Rlk, a novel protein tyrosine kinase with predominant expression in the T cell lineage.  |  Hu, Q., et al. 1995. J Biol Chem. 270: 1928-34. PMID: 7829530
9. TXK, a novel human tyrosine kinase expressed in T cells shares sequence identity with Tec family kinases and maps to 4p12.  |  Haire, RN., et al. 1994. Hum Mol Genet. 3: 897-901. PMID: 7951233
10. BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2.  |  Tamagnone, L., et al. 1994. Oncogene. 9: 3683-8. PMID: 7970727
11. Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes.  |  Thomas, JD., et al. 1993. Science. 261: 355-8. PMID: 8332900
12. Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells.  |  Yamada, N., et al. 1993. Biochem Biophys Res Commun. 192: 231-40. PMID: 8476425

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tec Antibody (G-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Tec Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tec Antibody (G-5) Specifications

Alternate Product Name: Tec

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 111-200

Epitope Target: Tec

Epitope Species: human

Product Reactivity/Detection: Tec

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Tec Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin