TDP1 Antibody (C-3): sc-365674

TDP1 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TDP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TDP1 (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tyrosyl-DNA phosphodiesterase 1 (TDP1) plays a crucial role in DNA repair by catalyzing the hydrolysis of phosphodiester bonds between tyrosine residues and DNA 3′-phosphates, which is essential for maintaining genomic stability. TDP1 is particularly important in the repair of DNA damage caused by free radicals, as TDP1 also removes glycolate from single-stranded DNA containing a 3′-phosphoglycolate, thereby facilitating the repair of double-strand breaks. The presence of a unique HKD signature motif, characterized by highly conserved lysine and histidine residues, classifies TDP1 within the phospholipase D superfamily, highlighting evolutionary significance. The hydrolytic reaction catalyzed by TDP1 involves a phosphoryl transfer mechanism that is common to all members of this superfamily. Loss-of-function mutations in TDP1 can lead to severe neurological disorders, such as spinocerebellar ataxia with axonal neuropathy, by disrupting DNA transcription and inducing apoptosis in postmitotic neurons, underscoring TDP1′s vital role in cellular health and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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TDP1 Antibody (C-3) References:

1. The tyrosyl-DNA phosphodiesterase Tdp1 is a member of the phospholipase D superfamily.  |  Interthal, H., et al. 2001. Proc Natl Acad Sci U S A. 98: 12009-14. PMID: 11572945
2. Conversion of phosphoglycolate to phosphate termini on 3' overhangs of DNA double strand breaks by the human tyrosyl-DNA phosphodiesterase hTdp1.  |  Inamdar, KV., et al. 2002. J Biol Chem. 277: 27162-8. PMID: 12023295
3. Mutation of TDP1, encoding a topoisomerase I-dependent DNA damage repair enzyme, in spinocerebellar ataxia with axonal neuropathy.  |  Takashima, H., et al. 2002. Nat Genet. 32: 267-72. PMID: 12244316
4. Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures.  |  Davies, DR., et al. 2002. J Mol Biol. 324: 917-32. PMID: 12470949

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TDP1 Antibody (C-3) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-TDP1 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TDP1 Antibody (C-3) Specifications

Alternate Product Name: TDP1

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 309-608

Epitope Target: TDP1

Epitope Species: human

Product Reactivity/Detection: TDP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for TDP1 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin