TDG CRISPR/Cas9 KO Plasmid (m): sc-423321
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TDG CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TDG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TDG Antibody (D-11): sc-376652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TDG CRISPR/Cas9 KO Plasmid (m) | sc-423321 | 20 µg | $397.00 |
Overview
Mouse TDG (thymine DNA glycosylase) is a base excision repair enzyme that recognizes and excises thymine or uracil mispaired with guanine, helping resolve G:T mismatches arising from deamination and replication errors. TDG also participates in active DNA demethylation by processing oxidized 5-methylcytosine derivatives generated by TET enzymes, linking it to epigenetic regulation and chromatin-associated transcriptional control. Through these activities, TDG contributes to genome stability, maintenance of DNA methylation patterns, and cellular responses to DNA damage. Altered TDG function has been associated with dysregulated epigenetic states and mutational processes that are relevant to studies of cancer biology, developmental programs, and inflammation-related genome maintenance.
TDG CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tdg gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tdg together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tdg open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TDG protein expression.
This CRISPR knockout system enables efficient generation of Tdg-deficient cell models for investigation of TDG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tdg exon(s) critical for TDG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tdg genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TDG CRISPR/Cas9 KO Plasmid (m) and TDG CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tdg locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TDG HDR Plasmid (m) and TDG HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tdg homology arms to support homology-directed repair at defined Tdg target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.