TCR α/β Antibody (WT31): sc-20078

TCR α/β Antibody (WT31) is a mouse monoclonal IgG1 kappa light chain antibody that detects the TCR alpha/beta protein of human origin by flow cytometry (FCM). Anti-TCR α/β antibody (WT31) is available in both non-conjugated and various conjugated forms, including agarose, biotin, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) plays a crucial role in the immune response by recognizing foreign antigens and initiating intracellular signaling that activates T cells, transitioning them from a dormant state to an active one. The TCR is a heterodimer composed of either alpha and beta or gamma and delta chains, with approximately 95% of circulating T cells expressing the alpha/beta heterodimer. This receptor is essential for T cell activation and is expressed on T helper and T cytotoxic cells, which can be differentiated by their expression of CD4 and CD8 co-receptors. CD4, a membrane glycoprotein with four extracellular immunoglobulin-like domains, enhances the interaction between the TCR and class II major histocompatibility complex (MHC) molecules presented by antigen-presenting cells, thereby increasing the avidity of the immune response. Understanding the function of the TCR and its interactions is vital for developing targeted immunotherapies and enhancing vaccine efficacy.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TCR α/β Antibody (WT31) References:

1. Adaptor proteins and T-cell antigen receptor signaling.  |  Samelson, LE. 1999. Prog Biophys Mol Biol. 71: 393-403. PMID: 10354706
2. T cell antigen receptor (TCR) transmembrane peptides colocalize with TCR, not lipid rafts, in surface membranes.  |  Wang, XM., et al. 2002. Cell Immunol. 215: 12-9. PMID: 12142032
3. Novel anti-CD4 monoclonal antibodies separate human immunodeficiency virus infection and fusion of CD4+ cells from virus binding.  |  Healey, D., et al. 1990. J Exp Med. 172: 1233-42. PMID: 1698911
4. The immunobiology of T cells with invariant gamma delta antigen receptors.  |  Allison, JP. and Havran, WL. 1991. Annu Rev Immunol. 9: 679-705. PMID: 1832874
5. Signal transduction by the T cell antigen receptor.  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
6. Identification of the residues in human CD4 critical for the binding of HIV.  |  Arthos, J., et al. 1989. Cell. 57: 469-81. PMID: 2541915
7. Structure and expression of the human and mouse T4 genes.  |  Maddon, PJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 9155-9. PMID: 3501122
8. Regulation of T-cell antigen receptor (TCR) alpha-chain expression by TCR beta-chain transcripts.  |  Chung, S. and Strominger, JL. 1995. Proc Natl Acad Sci U S A. 92: 3712-6. PMID: 7731970
9. The interaction between CD4 and MHC class II molecules and its effect on T cell function.  |  Vignali, DA. 1994. Behring Inst Mitt. 133-47. PMID: 7998907
10. T cell receptor interaction with peptide/major histocompatibility complex (MHC) and superantigen/MHC ligands is dominated by antigen.  |  Ehrich, EW., et al. 1993. J Exp Med. 178: 713-22. PMID: 8393480
11. Distinct roles for CD4 and CD8 as co-receptors in antigen receptor signalling.  |  Julius, M., et al. 1993. Immunol Today. 14: 177-83. PMID: 8499078
12. Crucial function of the pre-T-cell receptor (TCR) in TCR beta selection, TCR beta allelic exclusion and alpha beta versus gamma delta lineage commitment.  |  von Boehmer, H., et al. 1998. Immunol Rev. 165: 111-9. PMID: 9850856

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCR α/β Antibody (WT31) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TCR α/β Antibody (WT31) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCR α/β Antibody (WT31) Specifications

Alternate Product Name: T Cell Receptor

Product Clone Name: WT31

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: TCR α/β

Epitope Species: human

Product Reactivity/Detection: the conformational epitope formed by T-cell receptor (TCR) for antigen and the CD3 epsilon chain

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TCR α/β Antibody (WT31): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TCR β antibody is TCR β Antibody (G-11): sc-5277.