TCR α Antibody (H-1): sc-515719

TCR α Antibody (H-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects TCR alpha protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TCR alpha (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) plays a crucial role in the immune response by recognizing foreign antigens and initiating intracellular signaling that activates T cells, transitioning them from a dormant state to an active one. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains, with approximately 95% of circulating T cells expressing the alpha/beta heterodimer, while the remaining 2-5% express the gamma/delta variant. TCR′s functionality is enhanced by association with CD3 chains and the CD4 or CD8 co-receptors, which are essential for effective signal transduction. T helper cells, characterized by CD4 expression, and T cytotoxic cells, marked by CD8 expression, are vital components of the adaptive immune system. CD4, also known as T4 or Leu 3, is a membrane glycoprotein featuring four extracellular immunoglobulin-like domains, which facilitates the binding of TCR to class II major histocompatibility complex (MHC) molecules presented by antigen-presenting cells. This interaction is critical for the activation of T helper cells, as CD4 enhances the avidity of the TCR-MHC complex, thereby amplifying the immune response.

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TCR α Antibody (H-1) References:

1. Competitive displacement of pT alpha by TCR-alpha during TCR assembly prevents surface coexpression of pre-TCR and alpha beta TCR.  |  Trop, S., et al. 2000. J Immunol. 165: 5566-72. PMID: 11067911
2. Accessibility control of T cell receptor gene rearrangement in developing thymocytes. The TCR alpha/delta locus.  |  Krangel, MS., et al. 2000. Immunol Res. 22: 127-35. PMID: 11339350
3. Predominant role of T cell receptor (TCR)-alpha chain in forming preimmune TCR repertoire revealed by clonal TCR reconstitution system.  |  Yokosuka, T., et al. 2002. J Exp Med. 195: 991-1001. PMID: 11956290
4. Consequences of self and foreign superantigen interaction with specific V beta elements of the murine TCR alpha beta.  |  Kappler, JW., et al. 1989. Cold Spring Harb Symp Quant Biol. 54 Pt 1: 401-7. PMID: 2534796
5. Dual TCR-α Expression on Mucosal-Associated Invariant T Cells as a Potential Confounder of TCR Interpretation.  |  Suliman, S., et al. 2022. J Immunol. 208: 1389-1395. PMID: 35246495
6. Tnpo3 controls splicing of the pre-mRNA encoding the canonical TCR α chain of iNKT cells.  |  Iwanami, N., et al. 2023. Nat Commun. 14: 3645. PMID: 37339974
7. Expression of CD11a and CD45R isoforms defines distinct subsets of CD8+ TCR alpha beta and TCR gamma delta CTL in vivo.  |  Hedlund, G., et al. 1995. Immunol Rev. 146: 82-94. PMID: 7493762
8. Early molecular events induced by T cell receptor (TCR) signaling in immature CD4+ CD8+ thymocytes: increased synthesis of TCR-alpha protein is an early response to TCR signaling that compensates for TCR-alpha instability, improves TCR assembly, and parallels other indicators of positive selection.  |  Kearse, KP., et al. 1995. J Exp Med. 181: 193-202. PMID: 7528767
9. Stoichiometry of the T cell antigen receptor (TCR) complex: each TCR/CD3 complex contains one TCR alpha, one TCR beta, and two CD3 epsilon chains.  |  Punt, JA., et al. 1994. J Exp Med. 180: 587-93. PMID: 8046335
10. Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 epsilon/gamma dimers associate indistinctly with both TCR alpha and TCR beta chains. Evidence for a double TCR heterodimer model.  |  San José, E., et al. 1998. Eur J Immunol. 28: 12-21. PMID: 9485181

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCR α Antibody (H-1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-TCR α Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCR α Antibody (H-1) Specifications

Alternate Product Name: T Cell Receptor

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-142

Epitope Target: TCR α

Epitope Species: human

Product Reactivity/Detection: TCR α C region

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for TCR α Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TCR α antibody is TCR α Antibody (H28-710): sc-101410.