TCP-1 ζ Antibody (F-4): sc-514466
- TCP-1 ζ Antibody (F-4) is a mouse monoclonal IgG1 κ TCP-1 ζ antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 342-531 mapping at the C-terminus of TCP-1 ζ of human origin
- TCP-1 zeta Antibody (F-4) is recommended for detection of TCP-1 ζ and TCP-1 ζ2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TCP-1 zeta Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TCP-1 ζ Antibody (F-4) for WB applications. These reagents are now offered in bundles with TCP-1 ζ Antibody (F-4) (see ordering information below).
TCP-1 ζ Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TCP-1 zeta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TCP-1 ζ (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1, also known as t complex polypeptide 1, is a crucial component of the chaperonin containing TCP-1 (CCT) complex, which is essential for proper folding of cytosolic proteins, particularly actins and tubulins. Located in the eukaryotic cytosol, CCT complex consists of eight distinct subunits, each encoded by different genes, and includes two isoforms of the zeta subunit: TCP-1 ζ1 and TCP-1 ζ2. TCP-1′s ability to assist in folding these vital proteins is significant, as misfolded actins and tubulins can lead to various cellular dysfunctions and diseases. TCP-1 was initially identified in mice, where studies linked TCP-1 to tail-less and embryonic lethal phenotypes, and homologous sequences have been found in several other species, underscoring evolutionary importance. Studies of TCP-1 in yeast demonstrate function as a molecular chaperone for actin and tubulin, highlighting conserved roles across different organisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TCP-1 ζ Antibody (F-4) References:
- Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins. | Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
- Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress. | Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
- Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. | Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
- Defining the eukaryotic cytosolic chaperonin-binding sites in human tubulins. | Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
- Cucumber T-complex protein. Molecular cloning, bacterial expression and characterization within a 22-S cytosolic complex in cotyledons and hypocotyls. | Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
- A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development. | Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCP-1 ζ Antibody (F-4) | sc-514466 | 200 µg/ml | $322.00 | |||
TCP-1 ζ Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-527905 | 200 µg Ab; 10 µg BP | $361.00 | |||
TCP-1 ζ Antibody (F-4): m-IgG1 BP-HRP Bundle | sc-533278 | 200 µg Ab; 20 µg BP | $361.00 | |||
TCP-1 ζ Antibody (F-4) AC | sc-514466 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TCP-1 ζ Antibody (F-4) HRP | sc-514466 HRP | 200 µg/ml | $322.00 | |||
TCP-1 ζ Antibody (F-4) FITC | sc-514466 FITC | 200 µg/ml | $336.00 | |||
TCP-1 ζ Antibody (F-4) PE | sc-514466 PE | 200 µg/ml | $349.00 | |||
TCP-1 ζ Antibody (F-4) Alexa Fluor® 488 | sc-514466 AF488 | 200 µg/ml | $364.00 | |||
TCP-1 ζ Antibody (F-4) Alexa Fluor® 546 | sc-514466 AF546 | 200 µg/ml | $364.00 | |||
TCP-1 ζ Antibody (F-4) Alexa Fluor® 594 | sc-514466 AF594 | 200 µg/ml | $364.00 | |||
TCP-1 ζ Antibody (F-4) Alexa Fluor® 647 | sc-514466 AF647 | 200 µg/ml | $364.00 | |||
TCP-1 ζ Antibody (F-4) Alexa Fluor® 680 | sc-514466 AF680 | 200 µg/ml | $364.00 | |||
TCP-1 ζ Antibody (F-4) Alexa Fluor® 790 | sc-514466 AF790 | 200 µg/ml | $364.00 |