TCP-1 η Antibody (A-8): sc-271951

TCP-1 η Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects TCP-1 eta in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TCP-1 eta antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1 eta, also known as t complex polypeptide 1, is a crucial subunit of the chaperonin containing TCP-1 (CCT) complex, which is essential for the proper folding of cytosolic proteins, particularly actins and tubulins. This complex is composed of eight distinct subunits, each encoded by different genes, and plays a vital role in maintaining cellular function by ensuring proteins achieve their correct conformation. TCP-1 eta′s importance is underscored by involvement in critical cellular processes; mutations or dysfunctions can lead to severe developmental issues, as evidenced by identification in mouse models exhibiting tail-less and embryonic lethal phenotypes. Additionally, homologous sequences of TCP-1 eta have been found across various species, highlighting evolutionary conservation and significance in protein homeostasis.

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TCP-1 η Antibody (A-8) References:

1. Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins.  |  Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
2. Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress.  |  Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
3. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.  |  Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
4. Defining the eukaryotic cytosolic chaperonin-binding sites in human tubulins.  |  Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
5. Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction with the Viral Hexon Protein.  |  Gao, J., et al. 2019. Viruses. 11: PMID: 30691230
6. Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.  |  Huang, X., et al. 2022. Aging (Albany NY). 14: 747-769. PMID: 35073517
7. A hierarchical assembly pathway directs the unique subunit arrangement of TRiC/CCT.  |  Betancourt Moreira, K., et al. 2023. Mol Cell. 83: 3123-3139.e8. PMID: 37625406
8. STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation.  |  Chen, Y., et al. 2024. Nat Commun. 15: 44. PMID: 38168070
9. Serum biomarkers in patients with drug-resistant epilepsy: a proteomics-based analysis.  |  Ma, M., et al. 2024. Front Neurol. 15: 1383023. PMID: 38585359
10. Cucumber T-complex protein. Molecular cloning, bacterial expression and characterization within a 22-S cytosolic complex in cotyledons and hypocotyls.  |  Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
11. The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproduction.  |  Cyrne, L., et al. 1996. FEBS Lett. 383: 277-83. PMID: 8925913
12. A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development.  |  Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCP-1 η Antibody (A-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TCP-1 η Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCP-1 η Antibody (A-8) Specifications

Alternate Product Name: TCP-1 η

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 474-512

Epitope Target: TCP-1 η

Epitope Species: human

Product Reactivity/Detection: TCP-1 η

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TCP-1 η Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin