TCP-1 ε Antibody (G-3): sc-376188

TCP-1 ε Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TCP-1 epsilon protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TCP-1 ε (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1, also known as t complex polypeptide 1, is a crucial component of the chaperonin containing TCP-1 (CCT) complex, which is essential for proper folding of cytosolic proteins, particularly actins and tubulins. Located in the eukaryotic cytosol, CCT complex is composed of eight distinct subunits, each encoded by different genes, and plays a vital role in cellular function by ensuring proteins achieve their correct three-dimensional structures. TCP-1′s importance in protein folding is underscored by involvement in various cellular processes, including cell division and motility, making TCP-1 a key player in maintaining cellular integrity and function. TCP-1 was initially identified in mice, where TCP-1 was linked to tail-less and embryonic lethal phenotypes, and homologous sequences have been found in several other species, highlighting evolutionary significance. TCP-1′s ability to assist in folding of actin and tubulin underscores TCP-1′s critical role in cytoskeletal dynamics, which are essential for numerous cellular activities.

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TCP-1 ε Antibody (G-3) References:

1. Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins.  |  Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
2. Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress.  |  Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
3. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.  |  Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
4. Defining the eukaryotic cytosolic chaperonin-binding sites in human tubulins.  |  Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
5. Nested allosteric interactions in the cytoplasmic chaperonin containing TCP-1.  |  Kafri, G., et al. 2001. Protein Sci. 10: 445-9. PMID: 11266630
6. Assembly of the SMRT-histone deacetylase 3 repression complex requires the TCP-1 ring complex.  |  Guenther, MG., et al. 2002. Genes Dev. 16: 3130-5. PMID: 12502735
7. CCT5 interacts with cyclin D1 promoting lung adenocarcinoma cell migration and invasion.  |  Meng, Y., et al. 2021. Biochem Biophys Res Commun. 567: 222-229. PMID: 34217974
8. Recognition of the CCT5 di-Glu degron by CRL4DCAF12 is dependent on TRiC assembly.  |  Pla-Prats, C., et al. 2023. EMBO J. 42: e112253. PMID: 36715408
9. The Chaperone Protein Cct5 is Essential for Hematopoietic Stem Cell Maintenance.  |  Li, L., et al. 2024. Stem Cell Rev Rep. 20: 769-778. PMID: 38153635
10. Increased CCT5 expression is a potential unfavourable factor promoting the growth of nasopharyngeal carcinoma.  |  Wu, S. and Peng, L. 2024. J Int Med Res. 52: 3000605241271754. PMID: 39286844
11. Cucumber T-complex protein. Molecular cloning, bacterial expression and characterization within a 22-S cytosolic complex in cotyledons and hypocotyls.  |  Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
12. A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development.  |  Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCP-1 ε Antibody (G-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TCP-1 ε Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCP-1 ε Antibody (G-3) Specifications

Alternate Product Name: TCP-1 ε

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 111-390

Epitope Target: TCP-1 ε

Epitope Species: human

Product Reactivity/Detection: TCP-1 ε

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TCP-1 ε Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin