Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TCL-1B CRISPR/Cas9 KO Plasmid (h): sc-407297
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TCL-1B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TCL-1B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TCL-1B Antibody (H-3): sc-365169
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCL-1B CRISPR/Cas9 KO Plasmid (h) | sc-407297 | 20 µg | $397.00 |
Overview
TCL1B encodes TCL-1B, a member of the TCL1 family implicated in modulation of intracellular signaling that governs lymphoid cell growth and survival. TCL-1 family proteins have been linked to regulation of AKT/PKB-dependent pathways and downstream transcriptional programs that influence proliferation, apoptosis, and metabolic adaptation. Aberrant expression of TCL1 family members is frequently studied in the context of hematologic malignancies and dysregulated B- and T-cell biology, where altered signaling thresholds can contribute to oncogenic transformation and disease progression. As a result, TCL1B is used as a molecular handle to dissect signaling network rewiring and lineage-specific vulnerabilities in immune and cancer cell models.
TCL-1B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TCL1B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TCL1B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TCL1B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TCL-1B protein expression.
This CRISPR knockout system enables efficient generation of TCL1B-deficient cell models for investigation of TCL-1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TCL1B exon(s) critical for TCL-1B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TCL1B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TCL-1B CRISPR/Cas9 KO Plasmid (h) and TCL-1B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TCL1B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TCL-1B HDR Plasmid (h) and TCL-1B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TCL1B homology arms to support homology-directed repair at defined TCL1B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.