TCF-1 Antibody (C-5): sc-271453

TCF-1 Antibody (C-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects TCF-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TCF-1 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCF-1 protein, also known as transcription factor 7, plays a crucial role in lymphoid cell development as a DNA-binding transcriptional activator. TCF-1 is activated through the Wnt signaling pathway, which is essential for regulating gene expression during cell differentiation and proliferation. TCF-1 contains a high mobility group (HMG) box that facilitates binding to DNA, allowing regulation of Wnt target genes. TCF-1 exists in several splice variants, including TCF-1A, which share a conserved amino terminus but differ in carboxy terminal sequences, potentially influencing functional roles in various cellular contexts. TCF-1 interaction with β-catenin in the nucleus is a key aspect of function, as this complex promotes expression of genes involved in cell growth and development. Dysregulation of this pathway, often due to mutations in the adenomatous polyposis coli (APC) gene, can lead to aberrant β-catenin accumulation and increased activation of TCF target genes, frequently observed in colorectal cancer. anti-TCF-1 antibody (C-5) serves as a valuable tool for studying molecular mechanisms underlying lymphoid development and implications of Wnt signaling in cancer biology.

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TCF-1 Antibody (C-5) References:

1. Regnase-1 suppresses TCF-1+ precursor exhausted T-cell formation to limit CAR-T-cell responses against ALL.  |  Zheng, W., et al. 2021. Blood. 138: 122-135. PMID: 33690816
2. TCF-1 maintains CD8+ T cell stemness in tumor microenvironment.  |  Wen, S., et al. 2021. J Leukoc Biol. 110: 585-590. PMID: 34047386
3. TCF-1 controls Treg cell functions that regulate inflammation, CD8+ T cell cytotoxicity and severity of colon cancer.  |  Osman, A., et al. 2021. Nat Immunol. 22: 1152-1162. PMID: 34385712
4. Conventional type I dendritic cells maintain a reservoir of proliferative tumor-antigen specific TCF-1+ CD8+ T cells in tumor-draining lymph nodes.  |  Schenkel, JM., et al. 2021. Immunity. 54: 2338-2353.e6. PMID: 34534439
5. Interleukin-10 receptor signaling promotes the maintenance of a PD-1int TCF-1+ CD8+ T cell population that sustains anti-tumor immunity.  |  Hanna, BS., et al. 2021. Immunity. 54: 2825-2841.e10. PMID: 34879221
6. BCL6-dependent TCF-1+ progenitor cells maintain effector and helper CD4+ T cell responses to persistent antigen.  |  Xia, Y., et al. 2022. Immunity. 55: 1200-1215.e6. PMID: 35637103
7. TCF-1 regulates NKG2D expression on CD8 T cells during anti-tumor responses.  |  Harris, R., et al. 2023. Cancer Immunol Immunother. 72: 1581-1601. PMID: 36562825
8. Transcription factor TCF-1 regulates the functions, but not the development, of lymphoid tissue inducer subsets in different tissues.  |  Zheng, M., et al. 2023. Cell Rep. 42: 112924. PMID: 37540600
9. Sustained CD28 costimulation is required for self-renewal and differentiation of TCF-1+ PD-1+ CD8 T cells.  |  Humblin, E., et al. 2023. Sci Immunol. 8: eadg0878. PMID: 37624910
10. STING signaling promotes NK cell antitumor immunity and maintains a reservoir of TCF-1+ NK cells.  |  Lu, L., et al. 2023. Cell Rep. 42: 113108. PMID: 37708030

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCF-1 Antibody (C-5) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-TCF-1 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCF-1 Antibody (C-5) Specifications

Alternate Product Name: TCF7

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-118

Epitope Target: TCF-1

Epitope Species: human

Product Reactivity/Detection: TCF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for TCF-1 Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred TCF-1 antibody is TCF-1 Antibody (A-79): sc-101170.