Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TC-PTP CRISPR/Cas9 KO Plasmid (m): sc-422509
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TC-PTP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TC-PTP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TC-PTP Antibody (D-3): sc-398997
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TC-PTP CRISPR/Cas9 KO Plasmid (m) | sc-422509 | 20 µg | $397.00 |
Overview
Mouse Ptpn2 encodes T cell protein tyrosine phosphatase (TC-PTP), a non-receptor PTP that dephosphorylates key signaling intermediates to restrain cytokine and growth factor pathways. TC-PTP negatively regulates JAK/STAT signaling and can modulate insulin receptor and EGFR-associated phosphorylation events, shaping transcriptional programs that control proliferation, survival, and immune activation. Through these functions, Ptpn2 helps maintain immune homeostasis and epithelial barrier integrity, and its dysregulation has been linked to inflammatory and autoimmune phenotypes as well as altered tumor-associated signaling in experimental models. Accordingly, Ptpn2 is widely studied for mechanisms of cytokine responsiveness, T cell signaling thresholds, and inflammation-driven disease biology.
TC-PTP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ptpn2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ptpn2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ptpn2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TC-PTP protein expression.
This CRISPR knockout system enables efficient generation of Ptpn2-deficient cell models for investigation of TC-PTP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ptpn2 exon(s) critical for TC-PTP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ptpn2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TC-PTP CRISPR/Cas9 KO Plasmid (m) and TC-PTP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ptpn2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TC-PTP HDR Plasmid (m) and TC-PTP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ptpn2 homology arms to support homology-directed repair at defined Ptpn2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.