TBR-1 Antibody (G-5): sc-376258

TBR-1 Antibody (G-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects TBR-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TBR-1 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TBR-1, also known as T-brain-1, is a crucial transcription factor that plays a significant role in the development of the cerebral cortex during embryogenesis, where TBR-1 helps define distinct regions that contribute to the paleocortex, limbic cortex, and neocortex. TBR-1 expression is primarily restricted to postmitotic cells, highlighting TBR-1′s importance in neuronal differentiation and maturation. TBR-1 interacts with CASK/LIN-2, a membrane-associated guanylate kinase essential for epidermal growth factor receptor localization and signaling. This interaction is vital for forebrain development, as CASK can enter the nucleus and bind to specific DNA sequences in collaboration with TBR-1, thereby acting as a coactivator that induces the transcription of genes containing T-elements, including reelin. TBR-1′s ability to regulate gene expression in conjunction with CASK underscores TBR-1′s critical role in the intricate processes of brain development and function, making anti-TBR-1 antibody (G-5) an invaluable tool for researchers studying neurodevelopmental pathways and disorders.

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TBR-1 Antibody (G-5) References:

1. Nuclear translocation and transcription regulation by the membrane-associated guanylate kinase CASK/LIN-2.  |  Hsueh, YP., et al. 2000. Nature. 404: 298-302. PMID: 10749215
2. A starfish homolog of mouse T-brain-1 is expressed in the archenteron of Asterina pectinifera embryos: possible involvement of two T-box genes in starfish gastrulation.  |  Shoguchi, E., et al. 2000. Dev Growth Differ. 42: 61-8. PMID: 10831044
3. Pallial and subpallial derivatives in the embryonic chick and mouse telencephalon, traced by the expression of the genes Dlx-2, Emx-1, Nkx-2.1, Pax-6, and Tbr-1.  |  Puelles, L., et al. 2000. J Comp Neurol. 424: 409-38. PMID: 10906711
4. Overlapping expression of zebrafish T-brain-1 and eomesodermin during forebrain development.  |  Mione, M., et al. 2001. Mech Dev. 100: 93-7. PMID: 11118891
5. Cytoplasmic distribution of T-box transcription factor Tbr-1 in adult rodent brain.  |  Hong, CJ. and Hsueh, YP. 2007. J Chem Neuroanat. 33: 124-30. PMID: 17329080
6. Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality.  |  Huang, TN., et al. 2014. Nat Neurosci. 17: 240-7. PMID: 24441682
7. TBR1 regulates autism risk genes in the developing neocortex.  |  Notwell, JH., et al. 2016. Genome Res. 26: 1013-22. PMID: 27325115
8. Tbr1 Misexpression Alters Neuronal Development in the Cerebral Cortex.  |  Crespo, I., et al. 2022. Mol Neurobiol. 59: 5750-5765. PMID: 35781633
9. Shared and Distinct Functional Effects of Patient-Specific Tbr1 Mutations on Cortical Development.  |  Co, M., et al. 2022. J Neurosci. 42: 7166-7181. PMID: 35944998
10. Comparison of ITS-1 and TBR-1/2 primer sensitivity for the detection of Trypanosoma evansi local isolates in experimental rats using a polymerase chain reaction.  |  Suprihati, E., et al. 2022. Vet World. 15: 1772-1778. PMID: 36185509
11. Characterization of the TBR1 interactome: variants associated with neurodevelopmental disorders disrupt novel protein interactions.  |  Sollis, E., et al. 2023. Hum Mol Genet. 32: 1497-1510. PMID: 36579832
12. T-brain-1: a homolog of Brachyury whose expression defines molecularly distinct domains within the cerebral cortex.  |  Bulfone, A., et al. 1995. Neuron. 15: 63-78. PMID: 7619531

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TBR-1 Antibody (G-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-TBR-1 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TBR-1 Antibody (G-5) Specifications

Alternate Product Name: TBR-1

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-200

Epitope Target: TBR-1

Epitope Species: mouse

Product Reactivity/Detection: TBR-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for TBR-1 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent