Tau Antibody (Tau 46): sc-32274

Tau Antibody (Tau 46) is a mouse monoclonal IgG1 kappa light chain antibody developed to recognize purified native Tau protein of bovine origin, with its epitope mapped to the C-terminus of human Tau. Anti-Tau antibody (Tau 46) detects Tau and MAP-2 (280 kDa) from mouse, rat, and human species in applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF), and is predicted to recognize all six Tau isoforms as well as bovine Tau. Tau, also known as MAPT (microtubule-associated protein tau), or TAU, is a 758 amino acid protein that plays a crucial role in stabilizing microtubules within the neuronal cytoplasm, cytoskeleton, and cell membrane. The structure of Tau includes four microtubule-binding repeats, which are essential for promoting microtubule assembly and maintaining neuronal polarity, thereby supporting the intricate architecture and function of neurons. Tau′s structural flexibility allows adaptation to various cellular conditions, facilitating cytoskeletal plasticity necessary for processes such as axonal transport and synaptic function. Tau exists in multiple alternatively spliced isoforms, contributing to diverse functional roles in neuronal health. Post-translational modifications of Tau, including phosphorylation, polyubiquitination, and glycation, regulate activity and turnover, ensuring proper cytoskeletal dynamics. Dysregulation of Tau structure and function is implicated in several neurodegenerative diseases, including Alzheimer′s disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD), and progressive supranuclear palsy (PSP), highlighting the importance of studying Tau in these contexts. Tau monoclonal antibody (Tau 46) is available in both non-conjugated and various conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing researchers with versatile tools for investigating Tau′s structural and functional roles in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Tau Antibody (Tau 46) References:

1. Increased tau in the cerebrospinal fluid of patients with frontotemporal dementia and Alzheimer's disease.  |  Green, AJ., et al. 1999. Neurosci Lett. 259: 133-5. PMID: 10025576
2. Microtubule-associated protein-tau is a bifunctional predictor of endocrine sensitivity and chemotherapy resistance in estrogen receptor-positive breast cancer.  |  Andre, F., et al. 2007. Clin Cancer Res. 13: 2061-7. PMID: 17404087
3. A brief history of tau: the evolving view of the microtubule-associated protein tau in neurodegenerative diseases.  |  Lace, GL., et al. 2007. Clin Neuropathol. 26: 43-58. PMID: 17416103
4. Tau protein kinases: involvement in Alzheimer's disease.  |  Martin, L., et al. 2013. Ageing Res Rev. 12: 289-309. PMID: 22742992
5. Tauopathies and tau oligomers.  |  Takashima, A. 2013. J Alzheimers Dis. 37: 565-8. PMID: 23948895
6. Sources of extracellular tau and its signaling.  |  Avila, J., et al. 2014. J Alzheimers Dis. 40 Suppl 1: S7-S15. PMID: 24531154
7. Liquid-Liquid Phase Separation of Tau Protein in Neurobiology and Pathology.  |  Wegmann, S. 2019. Adv Exp Med Biol. 1184: 341-357. PMID: 32096048
8. Microtubule-associated protein tau epitopes are present in fiber lesions in diverse muscle disorders.  |  Lübke, U., et al. 1994. Am J Pathol. 145: 175-88. PMID: 7518193
9. A tau-like protein interacts with stress fibers and microtubules in human and rodent cultured cell lines.  |  Cross, D., et al. 1993. J Cell Sci. 105 (Pt 1): 51-60. PMID: 7689576
10. Regulation of mitochondrial pyruvate dehydrogenase activity by tau protein kinase I/glycogen synthase kinase 3beta in brain.  |  Hoshi, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 2719-23. PMID: 8610107
11. Differential phosphorylation of human tau isoforms containing three repeats by several protein kinases.  |  Singh, TJ., et al. 1996. Arch Biochem Biophys. 328: 43-50. PMID: 8638936
12. Protein phosphatase inhibitors induce modification of synapse structure and tau hyperphosphorylation in cultured rat hippocampal neurons.  |  Malchiodi-Albedi, F., et al. 1997. J Neurosci Res. 48: 425-38. PMID: 9185666

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tau Antibody (Tau 46) has 73 citations in a variety of scientific publications.

Primary antibodies like Anti-Tau Antibody (Tau 46) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tau Antibody (Tau 46) Specifications

Alternate Product Name: MAPT

Product Clone Name: Tau 46

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Tau

Epitope Species: bovine

Product Reactivity/Detection: Tau and MAP-2 (280 kDa)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 73 citations in various scientific publications

Available Conjugates/Forms for Tau Antibody (Tau 46): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin