Anti-Tau Antibody (A-10) recommended for detection of multiple Tau isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Tau Antibody (A-10): sc-390476

Anti-Tau Antibody (A-10) is rated 5.0 out of 5 by 4.
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Datasheets

    • Tau Antibody (A-10) is a mouse monoclonal IgG1 κ, cited in 24 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 716-735 near the C-terminus of Tau of human origin
    • Anti-Tau Antibody (A-10) is recommended for detection of multiple Tau isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-Tau Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Tau (A-10): sc-390476.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Tau Antibody (A-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Tau Antibody (A-10) (see ordering information below).
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The full length isoform of Tau is only 441 amino acids, correct?

Asked by: bjkrzesinski
Thank you for your question. The full length protein MAPT/Tau protein sequence published on Uniprot (see link below) is 758 amino acids. The displayed sequence is further processed into a mature form. This entry describes 9 isoforms produced by alternative splicing. https://www.uniprot.org/uniprot/P10636#sequences
Answered by: Technical Support
Date published: 2020-07-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Wow!! Outstanding Superb western and IHC data; far superior polyclonal alternatives
Date published: 2017-01-06
Rated 5 out of 5 by from Excellent IHC(P) signal Specific staining in human heart tissue at 1:50 dilution.
Date published: 2016-12-22
Rated 5 out of 5 by from Satisfactory Western Blot data of Tau expression Satisfactory Western Blot data of Tau expression in human brain tissue extract. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Produced positive Western Blot data of Tau Produced positive Western Blot data of Tau expression in SK-N-SH whole cell lysate and rat brain and mouse brain tissue extracts. -SCBT QC
Date published: 2014-08-24
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