Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TARM1 CRISPR/Cas9 KO Plasmid (m): sc-434122
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TARM1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TARM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TARM1 Antibody (B-4): sc-514218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TARM1 CRISPR/Cas9 KO Plasmid (m) | sc-434122 | 20 µg | $397.00 |
Overview
Tarm1 encodes TARM1, an immunoreceptor predominantly expressed on myeloid-lineage cells where it functions as a cell-surface signaling molecule that can modulate innate immune activation. TARM1 participates in receptor-driven pathways that influence cytokine production, inflammatory signaling cascades, and crosstalk between neutrophils, macrophages, and other leukocyte populations. Altered regulation of TARM1-linked signaling has been associated with immune dysregulation contexts relevant to inflammation and host defense, making it a useful target for mechanistic studies in mouse immunology models.
TARM1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tarm1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tarm1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tarm1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TARM1 protein expression.
This CRISPR knockout system enables efficient generation of Tarm1-deficient cell models for investigation of TARM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tarm1 exon(s) critical for TARM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tarm1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TARM1 CRISPR/Cas9 KO Plasmid (m) and TARM1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tarm1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TARM1 HDR Plasmid (m) and TARM1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tarm1 homology arms to support homology-directed repair at defined Tarm1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.