TAPP2 Antibody (A-7): sc-166351

TAPP2 Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects TAPP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TAPP2 antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Tandem PH (pleckstrin homology) domain-containing protein 2 (TAPP2) is a crucial cytoplasmic adaptor protein that plays a significant role in cellular signaling pathways, particularly in immune responses. TAPP2 is primarily located in the cytoplasm, with highest expression levels found in heart and kidney tissues, which underscores TAPP2′s importance in maintaining cellular functions in these vital organs. Upon stimulation by growth factors and activation of phosphoinositide 3-kinase, TAPP2 translocates to the plasma membrane, where TAPP2 accumulates in F-actin-rich membrane ruffles, facilitating critical interactions with phosphatidylinositol 3,4-bisphosphate through its C-terminal PH domain. This localization is essential for TAPP2′s role in regulating B and T cell activation, as TAPP2 enhances NFAT-dependent transcriptional activation and sustains calcium signaling during immune responses. The modulation of TAPP2 activity by Fc gamma receptor II and SHIP further highlights TAPP2′s regulatory functions in immune cell signaling, making TAPP2 a vital component in the orchestration of adaptive immunity.

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TAPP2 Antibody (A-7) References:

1. Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities.  |  Dowler, S., et al. 2000. Biochem J. 351: 19-31. PMID: 11001876
2. Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo.  |  Kimber, WA., et al. 2002. Biochem J. 361: 525-36. PMID: 11802782
3. TAPP1 and TAPP2 are targets of phosphatidylinositol 3-kinase signaling in B cells: sustained plasma membrane recruitment triggered by the B-cell antigen receptor.  |  Marshall, AJ., et al. 2002. Mol Cell Biol. 22: 5479-91. PMID: 12101241
4. Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1.  |  Kimber, WA., et al. 2003. Biochem J. 376: 525-35. PMID: 14516276
5. Two distinct waves of membrane-proximal B cell antigen receptor signaling differentially regulated by Src homology 2-containing inositol polyphosphate 5-phosphatase.  |  Krahn, AK., et al. 2004. J Immunol. 172: 331-9. PMID: 14688341
6. The phosphoinositol 3,4-bisphosphate-binding protein TAPP1 interacts with syntrophins and regulates actin cytoskeletal organization.  |  Hogan, A., et al. 2004. J Biol Chem. 279: 53717-24. PMID: 15485858
7. Role of the adaptor proteins Bam32, TAPP1 and TAPP2 in lymphocyte activation.  |  Allam, A. and Marshall, AJ. 2005. Immunol Lett. 97: 7-17. PMID: 15626471
8. The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane.  |  Ivetac, I., et al. 2005. Mol Biol Cell. 16: 2218-33. PMID: 15716355
9. Regulation of phosphoinositide 3-kinase signaling by oxidants: hydrogen peroxide selectively enhances immunoreceptor-induced recruitment of phosphatidylinositol (3,4) bisphosphate-binding PH domain proteins.  |  Cheung, SM., et al. 2007. Cell Signal. 19: 902-12. PMID: 17215104

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAPP2 Antibody (A-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TAPP2 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAPP2 Antibody (A-7) Specifications

Alternate Product Name: TAPP2

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 121-180

Epitope Target: TAPP2

Epitope Species: human

Product Reactivity/Detection: TAPP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TAPP2 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin