Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TAP2 CRISPR/Cas9 KO Plasmid (m): sc-423266
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TAP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAP2 Antibody (B-2): sc-515576
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAP2 CRISPR/Cas9 KO Plasmid (m) | sc-423266 | 20 µg | $397.00 |
Overview
Tap2 encodes the mouse transporter associated with antigen processing 2 (TAP2), an ATP-binding cassette (ABC) transporter that heterodimerizes with TAP1 to translocate proteasome-derived peptides from the cytosol into the endoplasmic reticulum for loading onto MHC class I molecules. This activity is central to antigen processing and presentation, linking ubiquitin–proteasome turnover, ER peptide loading, and CD8+ T cell immune surveillance. TAP2 function integrates with the peptide-loading complex and influences the quality and diversity of the MHC I immunopeptidome, shaping responses to infection and immune-mediated stress. Altered TAP2 activity has been associated with impaired MHC I surface expression and immune evasion phenotypes relevant to inflammatory biology and cancer immunology research.
TAP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tap2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tap2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tap2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAP2 protein expression.
This CRISPR knockout system enables efficient generation of Tap2-deficient cell models for investigation of TAP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tap2 exon(s) critical for TAP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tap2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TAP2 CRISPR/Cas9 KO Plasmid (m) and TAP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tap2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TAP2 HDR Plasmid (m) and TAP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tap2 homology arms to support homology-directed repair at defined Tap2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.