TAP2 Antibody (B-2): sc-515576

TAP2 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAP2 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAP2 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The transporter associated with antigen processing (TAP) plays a crucial role in immune response as TAP is integral to the major histocompatibility complex (MHC) class I antigen-presenting pathway. TAP is composed of two subunits, TAP1 and TAP2, which form stable dimers that create a translocation pore within endoplasmic reticulum (ER) membranes. This structure facilitates peptide movement from cytosol into ER lumen, where peptides are loaded onto MHC class I molecules for presentation to CD8+ T cells. TAP transporter is characterized by N-terminal transmembrane region that forms translocation pore and cytoplasmic peptide-binding pocket, along with several C-terminal nucleotide-binding domains essential for ATP binding and hydrolysis. These ATP-driven conformational changes are critical for effective peptide transport, underscoring TAP2′s importance in maintaining immune surveillance and response.

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TAP2 Antibody (B-2) References:

1. Nucleotide binding by TAP mediates association with peptide and release of assembled MHC class I molecules.  |  Knittler, MR., et al. 1999. Curr Biol. 9: 999-1008. PMID: 10508608
2. Membrane topology and dimerization of the two subunits of the transporter associated with antigen processing reveal a three-domain structure.  |  Vos, JC., et al. 1999. J Immunol. 163: 6679-85. PMID: 10586064
3. Pairing of the nucleotide binding domains of the transporter associated with antigen processing.  |  Lapinski, PE., et al. 2000. J Biol Chem. 275: 6831-40. PMID: 10702242
4. Genetic association between TAP1 and TAP2 polymorphisms and ankylosing spondylitis: a systematic review and meta-analysis.  |  Qian, Y., et al. 2017. Inflamm Res. 66: 653-661. PMID: 28405734
5. Correlations and Influence of TAP2 Genes Polymorphisms and Systemic Lupus Erythematosus Propensity.  |  Rezaieyazdi, Z., et al. 2021. Curr Rheumatol Rev. 17: 404-411. PMID: 33687897
6. Delayed Diagnosis of Chronic Necrotizing Granulomatous Skin Lesions due to TAP2 Deficiency.  |  Darazam, IA., et al. 2023. J Clin Immunol. 43: 217-228. PMID: 36227411
7. TAP2 Drives HLA-B∗13:01‒Linked Dapsone Hypersensitivity Syndrome Tolerance and Reactivity.  |  Sun, L., et al. 2023. J Invest Dermatol. 143: 722-730.e1. PMID: 37306379
8. LMP2 and TAP2 impair tumor growth and metastasis by inhibiting Wnt/β-catenin signaling pathway and EMT in cervical cancer.  |  Cheng, Z., et al. 2023. BMC Cancer. 23: 1128. PMID: 37986152
9. Characteristics of peptide and major histocompatibility complex class I/beta 2-microglobulin binding to the transporters associated with antigen processing (TAP1 and TAP2).  |  Androlewicz, MJ., et al. 1994. Proc Natl Acad Sci U S A. 91: 12716-20. PMID: 7809108
10. Evidence that transporters associated with antigen processing translocate a major histocompatibility complex class I-binding peptide into the endoplasmic reticulum in an ATP-dependent manner.  |  Androlewicz, MJ., et al. 1993. Proc Natl Acad Sci U S A. 90: 9130-4. PMID: 8415666
11. Multiple regions of the transporter associated with antigen processing (TAP) contribute to its peptide binding site.  |  Nijenhuis, M. and Hämmerling, GJ. 1996. J Immunol. 157: 5467-77. PMID: 8955196
12. Major histocompatibility complex class I molecules interact with both subunits of the transporter associated with antigen processing, TAP1 and TAP2.  |  Powis, SJ. 1997. Eur J Immunol. 27: 2744-7. PMID: 9368636

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAP2 Antibody (B-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-TAP2 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAP2 Antibody (B-2) Specifications

Alternate Product Name: TAP2

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 675-694

Epitope Target: TAP2

Epitope Species: mouse

Product Reactivity/Detection: TAP2

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for TAP2 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin