TAP Antibody (G-12): sc-28377

TAP Antibody (G-12) is a mouse monoclonal IgG1 antibody that detects TAP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAP, also known as tip-associating protein or NXF1, plays a crucial role in the nuclear export of unspliced viral RNAs, particularly those containing the constitutive transport element (CTE) found in type D retroviruses. Anti-TAP antibody (G-12) recognizes a protein essential for proper regulation of gene expression and viral replication, as TAP facilitates RNA transport from nucleus to cytoplasm, influencing overall efficiency of viral gene expression. TAP is part of a well-characterized mRNA export pathway and belongs to the multigene family of NXF proteins, which are evolutionarily conserved and characterized by a leucine-rich-repeat domain followed by a nuclear transport factor 2 (NTF2)-like domain. The structural features of TAP enable interaction with various RNA elements and other proteins involved in the export process, ensuring cellular machinery can effectively manage RNA transport and processing. TAP monoclonal antibody (G-12) serves as an invaluable tool for researchers studying RNA transport mechanisms and viral biology.

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TAP Antibody (G-12) References:

1. Nuclear export of mRNA by TAP/NXF1 requires two nucleoporin-binding sites but not p15.  |  Braun, IC., et al. 2002. Mol Cell Biol. 22: 5405-18. PMID: 12101235
2. TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD.  |  Saito, K., et al. 2004. Biochem Biophys Res Commun. 321: 291-7. PMID: 15358174
3. ICP27 recruits Aly/REF but not TAP/NXF1 to herpes simplex virus type 1 transcription sites although TAP/NXF1 is required for ICP27 export.  |  Chen, IH., et al. 2005. J Virol. 79: 3949-61. PMID: 15767397
4. The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable.  |  Johnson, LA., et al. 2009. J Virol. 83: 6335-46. PMID: 19369354
5. Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway.  |  Ote, I., et al. 2009. PLoS One. 4: e7882. PMID: 19924249
6. Evolutionary development of redundant nuclear localization signals in the mRNA export factor NXF1.  |  Zhang, ZC., et al. 2011. Mol Biol Cell. 22: 4657-68. PMID: 21965294
7. Structure and function of the porcine TAP protein and its inhibition by the viral immune evasion protein ICP47.  |  Chai, HH., et al. 2021. Int J Biol Macromol. 178: 514-526. PMID: 33662419
8. Evidence for Viral mRNA Export from Ebola Virus Inclusion Bodies by the Nuclear RNA Export Factor NXF1.  |  Wendt, L., et al. 2022. J Virol. 96: e0090022. PMID: 36040180
9. HnRNPA2B1 ISGylation Regulates m6A-Tagged mRNA Selective Export via ALYREF/NXF1 Complex to Foster Breast Cancer Development.  |  Jin, T., et al. 2024. Adv Sci (Weinh). 11: e2307639. PMID: 38626369
10. Cellular NS1-BP protein interacts with the mRNA export receptor NXF1 to mediate nuclear export of influenza virus M mRNAs.  |  Zhang, K., et al. 2024. J Biol Chem. 300: 107871. PMID: 39384042

The preferred TAP antibody is TAP Antibody (53H8): sc-32319.