Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tankyrase-1 CRISPR/Cas9 KO Plasmid (m): sc-423456
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tankyrase-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tankyrase-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tankyrase-1 Antibody (BL-2): sc-130422
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tankyrase-1 CRISPR/Cas9 KO Plasmid (m) | sc-423456 | 20 µg | $397.00 |
Overview
Mouse Tnks encodes Tankyrase-1, a PARP family poly(ADP-ribose) polymerase that modifies protein substrates to regulate their stability and interactions. Tankyrase-1 is a key node in Wnt/β-catenin signaling through PARylation-dependent control of AXIN turnover, and it also contributes to telomere maintenance, mitotic progression, and vesicle trafficking. By influencing cytoskeletal organization and genome integrity, Tankyrase-1 impacts pathways linked to aberrant proliferation and chromosomal instability, making it a relevant target for mechanistic studies of oncogenic signaling and DNA-associated processes in mouse models.
Tankyrase-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnks gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnks together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnks open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tankyrase-1 protein expression.
This CRISPR knockout system enables efficient generation of Tnks-deficient cell models for investigation of Tankyrase-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnks exon(s) critical for Tankyrase-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnks genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tankyrase-1 CRISPR/Cas9 KO Plasmid (m) and Tankyrase-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnks locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tankyrase-1 HDR Plasmid (m) and Tankyrase-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnks homology arms to support homology-directed repair at defined Tnks target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.