TANK Antibody (A-7): sc-166642
- TANK Antibody (A-7) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 126-425 mapping at the C-terminus of TANK of human origin
- recommended for detection of TANK of human origin by WB, IP, IF and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for TANK Antibody (A-7) for WB applications. This reagent is now offered in a bundle with TANK Antibody (A-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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TANK Antibody (A-7) is a mouse monoclonal IgG2b antibody that detects TANK in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TANK, or TRAF family member-associated NFKB activator, plays a crucial role in the regulation of the NFκB signaling pathway, which is vital for immune response, inflammation, and cell survival. Located primarily in the cytoplasm, TANK interacts with TRAF proteins, particularly TRAF2, to modulate the activation of NFκB. This interaction is significant because NFκB is a transcription factor that, when activated, translocates to the nucleus to initiate the expression of genes involved in immune and inflammatory responses. The regulatory carboxy-terminal domain of TANK keeps TANK inactive in unstimulated cells, ensuring that NFκB activation is tightly controlled. Upon binding to TRAF2, this inhibitory effect is lifted, allowing TANK to facilitate the activation of NFκB, thereby influencing various cellular processes. TANK antibody (A-7) is an essential tool for researchers studying the intricacies of immune signaling and the role of NFκB in disease mechanisms.
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TANK Antibody (A-7) References:
- NF-kappaB activation through IKK-i-dependent I-TRAF/TANK phosphorylation. | Nomura, F., et al. 2000. Genes Cells. 5: 191-202. PMID: 10759890
- TNFalpha- and IKKbeta-mediated TANK/I-TRAF phosphorylation: implications for interaction with NEMO/IKKgamma and NF-kappaB activation. | Bonif, M., et al. 2006. Biochem J. 394: 593-603. PMID: 16336209
- Lipopolysaccharide-mediated interferon regulatory factor activation involves TBK1-IKKepsilon-dependent Lys(63)-linked polyubiquitination and phosphorylation of TANK/I-TRAF. | Gatot, JS., et al. 2007. J Biol Chem. 282: 31131-46. PMID: 17823124
- The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1. | Zhang, W., et al. 2010. Mol Biol Cell. 21: 2500-13. PMID: 20484576
- TRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formation. | Maruyama, K., et al. 2012. J Biol Chem. 287: 29114-24. PMID: 22773835
- TRAF family member-associated NF-κB activator (TANK) induced by RANKL negatively regulates osteoclasts survival and function. | Wu, M., et al. 2012. Int J Biol Sci. 8: 1398-407. PMID: 23139637
- TRAF family member-associated NF-KB activator (TANK) gene polymorphism in chronic periodontitis and peri-implantitis patients. | Kadkhodazadeh, M., et al. 2012. J Long Term Eff Med Implants. 22: 127-36. PMID: 23428248
- NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. | Paul, S., et al. 2024. J Cell Biol. 223: PMID: 38059900
- Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. | Adriaenssens, E., et al. 2024. Nat Struct Mol Biol. 31: 1717-1731. PMID: 38918639
- I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. | Rothe, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 8241-6. PMID: 8710854
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TANK Antibody (A-7) | sc-166642 | 200 µg/ml | $316.00 | |||
TANK Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-534799 | 200 µg Ab; 40 µg BP | $354.00 |