TAG-72 Antibody (B72.3): sc-20042

TAG-72 Antibody (B72.3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAG-72 across various species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-TAG-72 antibody (B72.3) is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes. Tumor-associated glycoprotein 72 (TAG-72) is a high molecular weight glycoprotein found in cytoplasm and cell membrane of malignant cells, playing a crucial role in tumor biology. TAG-72 shows high expression in 80% of colorectal carcinomas but rarely appears in normal epithelial tissues or benign conditions, making TAG-72 a valuable cancer biomarker. TAG-72 expression is significant in low-grade mucoepidermoid carcinomas but absent in pure squamous cell mucoepidermoid carcinomas. TAG-72 remains weakly expressed or undetectable in normal adult tissues, except secretory endometrium, emphasizing TAG-72′s malignancy specificity. TAG-72 appears in various gynecologic cancers, including epithelial ovarian and endometrial carcinomas, while TAG-72 expression in prostatic adenocarcinomas negatively correlates with Gleason grade differentiation. TAG-72 prevalence in gastric, esophageal, pancreatic, and non-small cell lung carcinomas highlights TAG-72′s potential as a therapeutic target. TAG-72 proves valuable for purifying primary ovarian cancer cells from patient ascites, advancing research and treatment development.

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TAG-72 Antibody (B72.3) References:

1. TAG-72 expression and clinical outcome in primary breast cancer.  |  Pizzi, C., et al. 1999. Oncol Rep. 6: 1399-403. PMID: 10523719
2. Tumor associated glycoprotein-72 (TAG-72) levels in patients with non-malignant and malignant disease.  |  Filella, X., et al. 1992. Bull Cancer. 79: 271-7. PMID: 1392166
3. In vivo and in vitro clinical applications of monoclonal antibodies against TAG-72.  |  Colcher, D., et al. 1991. Int J Rad Appl Instrum B. 18: 395-401. PMID: 1864728
4. CA 72-4 radioimmunoassay for the detection of the TAG-72 carcinoma-associated antigen in serum of patients.  |  Gero, EJ., et al. 1989. J Clin Lab Anal. 3: 360-9. PMID: 2614571
5. The prognostic role of tumor associated glycoprotein 72 (TAG-72) in stage II and III colorectal adenocarcinoma.  |  Cho, J., et al. 2019. Pathol Res Pract. 215: 171-176. PMID: 30466765
6. TAG-72-Targeted α-Radionuclide Therapy of Ovarian Cancer Using 225Ac-Labeled DOTAylated-huCC49 Antibody.  |  Minnix, M., et al. 2021. J Nucl Med. 62: 55-61. PMID: 32620701
7. Engineered CAR-T cells targeting TAG-72 and CD47 in ovarian cancer.  |  Shu, R., et al. 2021. Mol Ther Oncolytics. 20: 325-341. PMID: 33614914
8. Survival Advantage Following TAG-72 Antigen-Directed Cancer Surgery in Patients With Colorectal Carcinoma: Proposed Mechanisms of Action.  |  Hitchcock, CL., et al. 2021. Front Oncol. 11: 731350. PMID: 34950576
9. Targeting TAG-72 in cutaneous T cell lymphoma.  |  Evtimov, VJ., et al. 2024. Heliyon. 10: e36298. PMID: 39263154
10. TAG-72 expression and its role in the biological evaluation of human colorectal cancer.  |  Guadagni, F., et al. 1996. Anticancer Res. 16: 2141-8. PMID: 8694534

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAG-72 Antibody (B72.3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TAG-72 Antibody (B72.3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAG-72 Antibody (B72.3) Specifications

Alternate Product Name: TAG-72

Product Clone Name: B72.3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: TAG-72

Epitope Species: human

Product Reactivity/Detection: TAG-72

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TAG-72 Antibody (B72.3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TAG-72 antibody is TAG-72 Antibody (CC49): sc-20043.