TAG-72 Antibody (B72.3): sc-20042
- TAG-72 Antibody (B72.3) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
- raised against a membrane-enriched fraction of a human breast carcinoma liver metastasis
- TAG-72 Antibody (B72.3) is recommended for detection of TAG-72 of broad species origin by WB, IP, IF, IHC(P) and FCM
- Anti-TAG-72 Antibody (B72.3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TAG-72 Antibody (B72.3) for WB and IHC(P) applications. These reagents are now offered in bundles with TAG-72 Antibody (B72.3) (see ordering information below).
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SEE ALSO...
TAG-72 Antibody (B72.3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAG-72 across various species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-TAG-72 antibody (B72.3) is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes. Tumor-associated glycoprotein 72 (TAG-72) is a high molecular weight glycoprotein found in cytoplasm and cell membrane of malignant cells, playing a crucial role in tumor biology. TAG-72 shows high expression in 80% of colorectal carcinomas but rarely appears in normal epithelial tissues or benign conditions, making TAG-72 a valuable cancer biomarker. TAG-72 expression is significant in low-grade mucoepidermoid carcinomas but absent in pure squamous cell mucoepidermoid carcinomas. TAG-72 remains weakly expressed or undetectable in normal adult tissues, except secretory endometrium, emphasizing TAG-72′s malignancy specificity. TAG-72 appears in various gynecologic cancers, including epithelial ovarian and endometrial carcinomas, while TAG-72 expression in prostatic adenocarcinomas negatively correlates with Gleason grade differentiation. TAG-72 prevalence in gastric, esophageal, pancreatic, and non-small cell lung carcinomas highlights TAG-72′s potential as a therapeutic target. TAG-72 proves valuable for purifying primary ovarian cancer cells from patient ascites, advancing research and treatment development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TAG-72 Antibody (B72.3) References:
- TAG-72 expression and clinical outcome in primary breast cancer. | Pizzi, C., et al. 1999. Oncol Rep. 6: 1399-403. PMID: 10523719
- Tumor associated glycoprotein-72 (TAG-72) levels in patients with non-malignant and malignant disease. | Filella, X., et al. 1992. Bull Cancer. 79: 271-7. PMID: 1392166
- In vivo and in vitro clinical applications of monoclonal antibodies against TAG-72. | Colcher, D., et al. 1991. Int J Rad Appl Instrum B. 18: 395-401. PMID: 1864728
- CA 72-4 radioimmunoassay for the detection of the TAG-72 carcinoma-associated antigen in serum of patients. | Gero, EJ., et al. 1989. J Clin Lab Anal. 3: 360-9. PMID: 2614571
- The prognostic role of tumor associated glycoprotein 72 (TAG-72) in stage II and III colorectal adenocarcinoma. | Cho, J., et al. 2019. Pathol Res Pract. 215: 171-176. PMID: 30466765
- TAG-72-Targeted α-Radionuclide Therapy of Ovarian Cancer Using 225Ac-Labeled DOTAylated-huCC49 Antibody. | Minnix, M., et al. 2021. J Nucl Med. 62: 55-61. PMID: 32620701
- Engineered CAR-T cells targeting TAG-72 and CD47 in ovarian cancer. | Shu, R., et al. 2021. Mol Ther Oncolytics. 20: 325-341. PMID: 33614914
- Survival Advantage Following TAG-72 Antigen-Directed Cancer Surgery in Patients With Colorectal Carcinoma: Proposed Mechanisms of Action. | Hitchcock, CL., et al. 2021. Front Oncol. 11: 731350. PMID: 34950576
- Targeting TAG-72 in cutaneous T cell lymphoma. | Evtimov, VJ., et al. 2024. Heliyon. 10: e36298. PMID: 39263154
- TAG-72 expression and its role in the biological evaluation of human colorectal cancer. | Guadagni, F., et al. 1996. Anticancer Res. 16: 2141-8. PMID: 8694534
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAG-72 Antibody (B72.3) | sc-20042 | 200 µg/ml | $322.00 | |||
TAG-72 Antibody (B72.3): m-IgG Fc BP-HRP Bundle | sc-528354 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAG-72 Antibody (B72.3): m-IgGκ BP-HRP Bundle | sc-520713 | 200 µg Ab, 40 µg BP | $361.00 | |||
TAG-72 Antibody (B72.3): m-IgG1 BP-HRP Bundle | sc-542886 | 200 µg Ab; 20 µg BP | $361.00 | |||
TAG-72 Antibody (B72.3) AC | sc-20042 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TAG-72 Antibody (B72.3) HRP | sc-20042 HRP | 200 µg/ml | $322.00 | |||
TAG-72 Antibody (B72.3) FITC | sc-20042 FITC | 200 µg/ml | $336.00 | |||
TAG-72 Antibody (B72.3) PE | sc-20042 PE | 200 µg/ml | $349.00 | |||
TAG-72 Antibody (B72.3) Alexa Fluor® 488 | sc-20042 AF488 | 200 µg/ml | $364.00 | |||
TAG-72 Antibody (B72.3) Alexa Fluor® 546 | sc-20042 AF546 | 200 µg/ml | $364.00 | |||
TAG-72 Antibody (B72.3) Alexa Fluor® 594 | sc-20042 AF594 | 200 µg/ml | $364.00 | |||
TAG-72 Antibody (B72.3) Alexa Fluor® 647 | sc-20042 AF647 | 200 µg/ml | $364.00 | |||
TAG-72 Antibody (B72.3) Alexa Fluor® 680 | sc-20042 AF680 | 200 µg/ml | $364.00 | |||
TAG-72 Antibody (B72.3) Alexa Fluor® 790 | sc-20042 AF790 | 200 µg/ml | $364.00 |