TAF II p43 Antibody (B-7): sc-398062
- TAF II p43 Antibody (B-7) is a mouse monoclonal IgG2a κ TAF II p43 antibody provided at 200 µg/ml
- raised against amino acids 40-310 mapping at the C-terminus of TAF II p43 of human origin
- TAF II p43 Antibody (B-7) is recommended for detection of TAF II p43 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TAF II p43 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TAF II p43 Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with TAF II p43 Antibody (B-7) (see ordering information below).
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TAF II p43 Antibody (B-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects TAF II p43 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TAF II p43 antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TAF II p43, also known as TAF8, TAFII43, or TBN, is a crucial 310 amino acid subunit of the TFIID complex, which is essential for transcription initiation. TAF II p43 contains a histone-fold domain vital for assembly of the preinitiation complex at the TATA promoter element. TAF II p43 mediates cellular response to various transcriptional activators and repressors, influencing gene expression. TAF II p43 localizes to either the nucleus or cytoplasm depending on cell developmental stage, playing a significant role in fibroblast differentiation and early embryonic survival. Ectopic expression of TAF II p43′s histone-fold domain can lead to dominant-negative mutations that hinder regulatory role in differentiation, potentially affecting developing cells. Alternative splicing events produce four distinct TAF II p43 isoforms, highlighting regulatory potential in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TAF II p43 Antibody (B-7) References:
- Positive and negative TAF(II) functions that suggest a dynamic TFIID structure and elicit synergy with traps in activator-induced transcription. | Guermah, M., et al. 2001. Mol Cell Biol. 21: 6882-94. PMID: 11564872
- A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. | Tora, L. 2002. Genes Dev. 16: 673-5. PMID: 11963920
- The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis. | Guermah, M., et al. 2003. Mol Cell. 12: 991-1001. PMID: 14580349
- The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners. | Soutoglou, E., et al. 2005. Mol Cell Biol. 25: 4092-104. PMID: 15870280
- Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly. | Pendergrast, PS., et al. 1996. J Virol. 70: 5025-34. PMID: 8764009
- Genomics and transcription analysis of human TFIID. | Purrello, M., et al. 1998. Oncogene. 16: 1633-8. PMID: 9569032
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF II p43 Antibody (B-7) | sc-398062 | 200 µg/ml | $322.00 | |||
TAF II p43 Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-530686 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAF II p43 Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-524206 | 200 µg Ab, 40 µg BP | $361.00 | |||
TAF II p43 Antibody (B-7): m-IgG2a BP-HRP Bundle | sc-547562 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAF II p43 Antibody (B-7) AC | sc-398062 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TAF II p43 Antibody (B-7) HRP | sc-398062 HRP | 200 µg/ml | $322.00 | |||
TAF II p43 Antibody (B-7) FITC | sc-398062 FITC | 200 µg/ml | $336.00 | |||
TAF II p43 Antibody (B-7) PE | sc-398062 PE | 200 µg/ml | $349.00 | |||
TAF II p43 Antibody (B-7) Alexa Fluor® 488 | sc-398062 AF488 | 200 µg/ml | $364.00 | |||
TAF II p43 Antibody (B-7) Alexa Fluor® 546 | sc-398062 AF546 | 200 µg/ml | $364.00 | |||
TAF II p43 Antibody (B-7) Alexa Fluor® 594 | sc-398062 AF594 | 200 µg/ml | $364.00 | |||
TAF II p43 Antibody (B-7) Alexa Fluor® 647 | sc-398062 AF647 | 200 µg/ml | $364.00 | |||
TAF II p43 Antibody (B-7) Alexa Fluor® 680 | sc-398062 AF680 | 200 µg/ml | $364.00 | |||
TAF II p43 Antibody (B-7) Alexa Fluor® 790 | sc-398062 AF790 | 200 µg/ml | $364.00 |