Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TAF II p135 CRISPR/Cas9 KO Plasmid (h): sc-404230
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAF II p135 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TAF II p135 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAF II p135 Antibody (22): sc-136093
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF II p135 CRISPR/Cas9 KO Plasmid (h) | sc-404230 | 20 µg | $397.00 |
Overview
TAF4 encodes TAF II p135, a core subunit of the TFIID complex that cooperates with TBP and other TAFs to initiate RNA polymerase II–dependent transcription. Through interactions with sequence-specific transcription factors and chromatin-associated regulators, TAF II p135 contributes to promoter recognition, preinitiation complex assembly, and control of cell state–dependent gene expression programs. TAF4 function is closely tied to chromatin remodeling and transcriptional coactivator networks, linking it to regulation of proliferation, differentiation, and stress-responsive transcription. Dysregulated TFIID/TAF activity and altered transcriptional initiation are frequently studied in the context of oncogenic transcriptional reprogramming and other disorders of gene regulation.
TAF II p135 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TAF4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TAF4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TAF4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAF II p135 protein expression.
This CRISPR knockout system enables efficient generation of TAF4-deficient cell models for investigation of TAF II p135 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TAF4 exon(s) critical for TAF II p135 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TAF4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TAF II p135 CRISPR/Cas9 KO Plasmid (h) and TAF II p135 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TAF4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TAF II p135 HDR Plasmid (h) and TAF II p135 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TAF4 homology arms to support homology-directed repair at defined TAF4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.