TAF II p135 Antibody (22): sc-136093

TAF II p135 Antibody (22) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAF II p135 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-TAF II p135 antibody (22) is available as the non-conjugated format. TAF II p135, also known as TAF4, TAF2C, TAF2C1, TAF4A, or TAFII130, is a crucial 1,085 amino acid subunit of the TFIID complex, which plays a vital role in the initiation of transcription by facilitating the assembly of the preinitiation complex at the TATA promoter element. TAF II p135 is primarily localized in the nucleus, where TAF II p135 interacts with various transcriptional activators and repressors, thereby mediating promoter responses. Notably, TAF II p135 contains a TAFH domain that enables binding to proteins with glutamine-rich domains, such as the transcription factor CREB. This interaction is significant as TAF II p135 may link to neurodegenerative diseases associated with polyglutamine expansions, including Dentatorubropallidoluysian atrophy, Huntington′s disease, and Spinocerebellar ataxia. TAF II p135′s ability to accelerate transcriptional activation in response to thyroid hormone and retinoic acid further underscores its importance in gene regulation and cellular function.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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TAF II p135 Antibody (22) References:

1. Isoform-specific interaction of HP1 with human TAFII130.  |  Vassallo, MF. and Tanese, N. 2002. Proc Natl Acad Sci U S A. 99: 5919-24. PMID: 11959914
2. Sp1 and TAFII130 transcriptional activity disrupted in early Huntington's disease.  |  Dunah, AW., et al. 2002. Science. 296: 2238-43. PMID: 11988536
3. Crystal structure of a subcomplex of human transcription factor TFIID formed by TATA binding protein-associated factors hTAF4 (hTAF(II)135) and hTAF12 (hTAF(II)20).  |  Werten, S., et al. 2002. J Biol Chem. 277: 45502-9. PMID: 12237304
4. Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis.  |  Cavusoglu, N., et al. 2003. Proteomics. 3: 217-23. PMID: 12601814
5. The intracellular localisation of TAF7L, a paralogue of transcription factor TFIID subunit TAF7, is developmentally regulated during male germ-cell differentiation.  |  Pointud, JC., et al. 2003. J Cell Sci. 116: 1847-58. PMID: 12665565
6. A role of the TATA box and the general co-activator hTAF(II)130/135 in promoter-specific trans-activation by simian virus 40 small t antigen.  |  Johannessen, M., et al. 2003. J Gen Virol. 84: 1887-1897. PMID: 12810884
7. The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis.  |  Guermah, M., et al. 2003. Mol Cell. 12: 991-1001. PMID: 14580349
8. A functional interaction between ATF7 and TAF12 that is modulated by TAF4.  |  Hamard, PJ., et al. 2005. Oncogene. 24: 3472-83. PMID: 15735663
9. Structural changes in TAF4b-TFIID correlate with promoter selectivity.  |  Liu, WL., et al. 2008. Mol Cell. 29: 81-91. PMID: 18206971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAF II p135 Antibody (22) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-TAF II p135 Antibody (22) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAF II p135 Antibody (22) Specifications

Alternate Product Name: TAF II p135

Product Clone Name: 22

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 454-565

Epitope Target: TAF II p135

Epitope Species: human

Product Reactivity/Detection: TAF II p135

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for TAF II p135 Antibody (22): N/A