T2R3 Antibody (E-12): sc-398489
- T2R3 Antibody (E-12) is a mouse monoclonal IgG1 λ T2R3 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 72-89 within an extracellular domain of T2R3 of human origin
- T2R3 Antibody (E-12) is recommended for detection of T2R3 of human origin, T2R37 of mouse origin and the corresponding rat homolog by WB, IP, IF and ELISA
- Anti-T2R3 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for T2R3 Antibody (E-12) for WB applications. This reagent is now offered in a bundle with T2R3 Antibody (E-12) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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T2R3 Antibody (E-12) is a mouse monoclonal IgG1 antibody that detects T2R3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). T2R3 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. T2R3, also known as taste receptor type 2 member 3 or TAS2R3, is a 316 amino acid multi-pass membrane protein that plays a crucial role in the perception of bitter tastes. T2R3 is part of the G-protein coupled receptor T2R family and is primarily expressed in taste receptor cells located in the tongue and palate epithelium, as well as in specific regions of the gastrointestinal tract, including the stomach and duodenum. T2R3 functions as a gustducin-coupled receptor, mediating responses to bitter compounds through a signaling pathway involving phospholipase C β2 and the calcium-regulated cation channel TRPM5. This signaling is essential for detecting potentially harmful substances, as bitter taste perception is closely linked to the avoidance of toxic compounds. T2R3 gene is located on human chromosome 7q34 and consists of approximately 1,101 bases, highlighting its evolutionary significance in taste perception and dietary choices.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
T2R3 Antibody (E-12) References:
- A novel family of mammalian taste receptors. | Adler, E., et al. 2000. Cell. 100: 693-702. PMID: 10761934
- T2Rs function as bitter taste receptors. | Chandrashekar, J., et al. 2000. Cell. 100: 703-11. PMID: 10761935
- A plethora of taste receptors. | Kinnamon, SC. 2000. Neuron. 25: 507-10. PMID: 10774719
- Identification of coding single-nucleotide polymorphisms in human taste receptor genes involving bitter tasting. | Ueda, T., et al. 2001. Biochem Biophys Res Commun. 285: 147-51. PMID: 11437385
- Molecular mechanisms of bitter and sweet taste transduction. | Margolskee, RF. 2002. J Biol Chem. 277: 1-4. PMID: 11696554
- Receptors for bitter and sweet taste. | Montmayeur, JP. and Matsunami, H. 2002. Curr Opin Neurobiol. 12: 366-71. PMID: 12139982
- Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. | Zhang, Y., et al. 2003. Cell. 112: 293-301. PMID: 12581520
- Evolution of bitter taste receptors in humans and apes. | Fischer, A., et al. 2005. Mol Biol Evol. 22: 432-6. PMID: 15496549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T2R3 Antibody (E-12) | sc-398489 | 200 µg/ml | $322.00 | |||
T2R3 Antibody (E-12): m-IgGλ BP-HRP Bundle | sc-524364 | 200 µg Ab, 40 µg BP | $361.00 | |||
T2R3 Antibody (E-12) AC | sc-398489 AC | 500 µg/ml, 25% agarose | $424.00 | |||
T2R3 Antibody (E-12) HRP | sc-398489 HRP | 200 µg/ml | $322.00 | |||
T2R3 Antibody (E-12) FITC | sc-398489 FITC | 200 µg/ml | $336.00 | |||
T2R3 Antibody (E-12) PE | sc-398489 PE | 200 µg/ml | $349.00 | |||
T2R3 Antibody (E-12) Alexa Fluor® 488 | sc-398489 AF488 | 200 µg/ml | $364.00 | |||
T2R3 Antibody (E-12) Alexa Fluor® 546 | sc-398489 AF546 | 200 µg/ml | $364.00 | |||
T2R3 Antibody (E-12) Alexa Fluor® 594 | sc-398489 AF594 | 200 µg/ml | $364.00 | |||
T2R3 Antibody (E-12) Alexa Fluor® 647 | sc-398489 AF647 | 200 µg/ml | $364.00 | |||
T2R3 Antibody (E-12) Alexa Fluor® 680 | sc-398489 AF680 | 200 µg/ml | $364.00 | |||
T2R3 Antibody (E-12) Alexa Fluor® 790 | sc-398489 AF790 | 200 µg/ml | $364.00 | |||
T2R3 (E-12) Neutralizing Peptide | sc-398489 P | 100 µg/0.5 ml | $69.00 |