Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
T-FABP CRISPR/Cas9 KO Plasmid (m): sc-423411
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T-FABP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the T-FABP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: T-FABP Antibody (B-4): sc-390196
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T-FABP CRISPR/Cas9 KO Plasmid (m) | sc-423411 | 20 µg | $397.00 |
Overview
Fabp9 encodes testis fatty acid–binding protein (T-FABP), a lipid chaperone implicated in intracellular trafficking of long-chain fatty acids and other hydrophobic ligands in the male germline. By regulating fatty acid uptake, sequestration, and delivery to metabolic enzymes and membranes, T-FABP is linked to lipid homeostasis pathways that influence mitochondrial β-oxidation, membrane remodeling, and redox balance during spermatogenesis. Altered FABP family activity is broadly associated with metabolic dysregulation and inflammatory signaling, and Fabp9 is commonly studied in the context of sperm development, motility, and fertility-related phenotypes. In mouse models, Fabp9 provides a tractable node to interrogate how germ cell lipid handling impacts reproductive biology and stress-responsive metabolic adaptation.
T-FABP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fabp9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fabp9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fabp9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish T-FABP protein expression.
This CRISPR knockout system enables efficient generation of Fabp9-deficient cell models for investigation of T-FABP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Fabp9 exon(s) critical for T-FABP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Fabp9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by T-FABP CRISPR/Cas9 KO Plasmid (m) and T-FABP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fabp9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by T-FABP HDR Plasmid (m) and T-FABP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fabp9 homology arms to support homology-directed repair at defined Fabp9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.