T-cadherin Antibody (E-9) recommended for detection of T-cadherin of mouse, rat and human origin by WB, IP, IF and ELISA

T-cadherin Antibody (E-9): sc-166875

T-cadherin Antibody (E-9) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-T-cadherin Antibody (E-9) is a mouse monoclonal IgG2b (kappa light chain) T-cadherin antibody provided at 200 µg/ml
    • raised against amino acids 300-425 mapping within an internal region of T-cadherin of human origin
    • Anti-T-cadherin Antibody (E-9) is recommended for detection of T-cadherin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine
    • Anti-T-cadherin Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of T-cadherin (E-9): sc-166875.
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What is the recommended fixation method for immunofluorescence staining with T-cadherin (E-9): sc-166875 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Very good effciency Very good efficiency (1:200), but multiple bands visible. Can be used more diluted than recommended.
Date published: 2017-11-28
Rated 3 out of 5 by from Immunofluorescence in neuroblastoma cells We used this antibody for staining neuroblastoma cells (SH-SY5Y) and the antibody worked, but the signal was weak.
Date published: 2017-02-01
Rated 5 out of 5 by from Worked in IF with rat brain tissues Worked in IF with rat brain tissues
Date published: 2015-04-06
Rated 4 out of 5 by from Good for methanol fixed IF Good for methanol fixed IF - worked in HeLa -SCBT QC
Date published: 2014-11-05
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