SYT Antibody (E-5): sc-374299

SYT Antibody (E-5) is a mouse monoclonal IgG2b antibody that detects SYT in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SYT, or synovial translocation protein, plays a crucial role as a transcriptional coactivator, facilitating the expression of genes involved in cell growth and differentiation, which is particularly important in the context of synovial sarcoma, a type of cancer that arises in soft tissues. SYT features a conserved amino terminal SNH domain and a carboxy terminal QPGY domain, the latter of which acts as a transcriptional activating sequence. SYT is involved in the t(x;18) translocation, leading to the formation of SYT-SSX fusion proteins that function as potent transcriptional activators, contributing to the oncogenic process in synovial sarcomas. These fusions are predominantly found in synovial sarcomas, which account for up to 10% of soft tissue sarcomas and can be classified into biphasic and monophasic subtypes based on the specific fusion partner, either SSX1 or SSX2. Anti-SYT antibody (E-5) detection of these critical fusion proteins makes this reagent an invaluable tool for researchers studying synovial sarcoma and its underlying molecular mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SYT Antibody (E-5) References:

1. Primary synovial sarcoma of the lung: a case report confirmed by molecular detection of SYT-SSX fusion gene transcripts.  |  Terasaki, H., et al. 2001. Jpn J Clin Oncol. 31: 212-6. PMID: 11450996
2. SYT-SSX fusion genes and prognosis in synovial sarcoma.  |  Mezzelani, A., et al. 2001. Br J Cancer. 85: 1535-9. PMID: 11720441
3. Detection of SYT-SSX fusion transcripts in archival synovial sarcomas by real-time reverse transcriptase-polymerase chain reaction.  |  Bijwaard, KE., et al. 2002. J Mol Diagn. 4: 59-64. PMID: 11826189
4. A novel type of SYT/SSX fusion: methodological and biological implications.  |  Törnkvist, M., et al. 2002. Mod Pathol. 15: 679-85. PMID: 12065783
5. The SYT-SSX1 fusion type of synovial sarcoma is associated with increased expression of cyclin A and D1. A link between t(X;18)(p11.2; q11.2) and the cell cycle machinery.  |  Xie, Y., et al. 2002. Oncogene. 21: 5791-6. PMID: 12173050
6. Primary pulmonary synovial sarcoma confirmed by molecular detection of SYT-SSX1 fusion gene transcripts: a case report and review of the literature.  |  Hosono, T., et al. 2005. Jpn J Clin Oncol. 35: 274-9. PMID: 15879502
7. SYT-SSX1 (synovial sarcoma translocated) regulates PIASy ligase activity to cause overexpression of NCOA3 protein.  |  Sun, Y., et al. 2011. J Biol Chem. 286: 18623-32. PMID: 21454665
8. Poorly differentiated pulmonary synovial sarcoma with SYT gene amplification: A case report.  |  Li, X., et al. 2019. Mol Clin Oncol. 10: 249-252. PMID: 30680203
9. Nuclear localization of SYT, SSX and the synovial sarcoma-associated SYT-SSX fusion proteins.  |  dos Santos, NR., et al. 1997. Hum Mol Genet. 6: 1549-58. PMID: 9285793
10. The SYT protein involved in the t(X;18) synovial sarcoma translocation is a transcriptional activator localised in nuclear bodies.  |  Brett, D., et al. 1997. Hum Mol Genet. 6: 1559-64. PMID: 9285794

The preferred SYT antibody is SYT Antibody (D-3): sc-390615.