Anti-SYP/Synaptophysin Antibody (H-8) recommended for detection of SYP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-SYP/Synaptophysin Antibody (H-8): sc-55507

Anti-SYP/Synaptophysin Antibody (H-8) is rated 4.2 out of 5 by 10.
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Datasheets

    • SYP/Synaptophysin Antibody (H-8) is a mouse monoclonal IgG1 κ, cited in 32 publications, provided at 200 µg/ml
    • raised against amino acids 221-313 of SYP of human origin
    • Anti-SYP/Synaptophysin Antibody (H-8) is recommended for detection of SYP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SYP/Synaptophysin Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SYP/Synaptophysin (H-8): sc-55507.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SYP/Synaptophysin Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with SYP/Synaptophysin Antibody (H-8) (see ordering information below).
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I am using SYP (H-8): sc-55507 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works in Mouse and Human We got this as a sample. Performed on FFPE human and mouse tissues. Sodium citrate 30 minutes at 1:250. Very easy and looks great!
Date published: 2017-11-17
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Very Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-25
Rated 4 out of 5 by from Great antibody Great antibody. It worked well without any problems.
Date published: 2015-10-07
Rated 4 out of 5 by from Various publishable data in WB using mouse Various publishable data in WB using mouse cerebral cortex and hippocampal tissues -SCBT Publication Review
Date published: 2015-06-30
Rated 4 out of 5 by from Publishable data in WB using rat brain Publishable data in WB using rat brain. -SCBT Publication Review
Date published: 2015-06-06
Rated 4 out of 5 by from Publishable data in IHC using human DAOY Publishable data in IHC using human DAOY,G401,central nervous system atypical teratoid rhabdoid tumor (AT/RT) -SCBT Publication Review
Date published: 2015-04-21
Rated 3 out of 5 by from Robust bands detected in WB using IMR Robust bands detected in WB using IMR-32 whole cell lysate and rat and mouse brain tissue extracts. -SCBT QC
Date published: 2015-04-17
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