SYP/Synaptophysin Antibody (H-8): sc-55507

SYP Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects synaptophysin (SYP) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SYP antibody (H-8) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Synaptophysin is a crucial integral membrane protein predominantly found in neurons and endocrine cells, where SYP (H-8) monoclonal antibody plays a vital role in the formation and recycling of synaptic vesicles. Synaptophysin is characterized by N-glycosylation and the ability to form hexameric complexes, which contribute to functioning as a large conductance channel. SYP contains four transmembrane domains and regulates membrane cholesterol, essential for vesicle formation. Synaptophysin interacts with synaptobrevin (VAMP), forming a complex upregulated during neuronal development, although not essential for the exocytosis process itself. The tail domain of brain myosin V forms a stable complex with synaptobrevin II and synaptophysin, which disassembles upon calcium ion influx during depolarization, highlighting the dynamic nature of synaptic vesicle cycling and SYP′s role in neurotransmission.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SYP/Synaptophysin Antibody (H-8) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-SYP/Synaptophysin Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SYP/Synaptophysin Antibody (H-8) Specifications

Alternate Product Name: Synaptophysin

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 221-313

Epitope Target: SYP

Epitope Species: human

Product Reactivity/Detection: SYP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for SYP/Synaptophysin Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SYP/Synaptophysin antibody is SYP/Synaptophysin Antibody (D-4): sc-17750.