Anti-Syntaxin 11 Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain) Syntaxin 11 antibody provided at 200 µg/ml
raised against amino acids 147-266 mapping near the C-terminus of Syntaxin 11 of human origin
Syntaxin 11 Antibody (A-4) is recommended for detection of Syntaxin 11 of human origin by WB, IP, IF and ELISA
Anti-Syntaxin 11 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Syntaxin 11 (A-4): sc-377121.
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Syntaxin 11 Antibody (A-4) is a high quality monoclonal Syntaxin 11 antibody (also designated Syntaxin 11 antibody) suitable for the detection of the Syntaxin 11 protein of human origin. Syntaxin 11 Antibody (A-4) is available as both the non-conjugated anti-Syntaxin 11 antibody form, as well as multiple conjugated forms of anti-Syntaxin 11 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain C-terminal hydrophobic domains that direct them to their respective intracellular compartments. Syntaxin 11, also known as STX11, FHL4, HLH4 or HPLH4 is a 287 amino acid protein that contains one t-SNARE coiled-coil homology domain and localizes to the membrane of the trans-golgi network. Interacting with SNAP 23 and VAMP, Syntaxin 11 functions to regulate protein transport between the trans-golgi network and late endosomes. Defects in the gene encoding Syntaxin 11 are the cause of familial hemophagocytic lymphohistiocytosis type 4 (FHL4), a genetically heterogeneous autosomal recessive disorder that is characterized by fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, seizures, cranial nerve deficits and ataxia.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
For best results, do I dilute Syntaxin 11 antibody in 5% Milk in TBST or 5% BSA in TBST?
Asked by: abed
Thank you for your question. We recommend blocking, and incubating both primary and secondary antibody in 5% nonfat milk in TBST (0.05% Tween-20).
Answered by: Technical Support
Date published: 2019-07-23
It says recommended for IF. Do you have any images?
Asked by: alexmm
Thank you for your question. We have not yet tested this specific mouse monoclonal, Syntaxin 11, sc-377121, for IF so we do not have any specific recommendations we can offer at this time for this research application. However, the antibody has been tested in Western blot and ELISA, so we believe it should also work for IF based on experience and in house testing. The antibody is warrantied for IF use, so if it does not perform as expected in IF applications, we will be happy to replace, credit or refund your purchase! If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-04-26
What is the recommended starting dilution for Western blot for sc-377121: Syntaxin 11 (A-4) monoclonal antibody?
Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 5 out of
Produced positive Western blot data of SyntaxinProduced positive Western blot data of Syntaxin 11 expression in human spleen tissue extract and human PBL whole cell lysate . -SCBT QC
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