Synoviolin Antibody (4H4): sc-293484

Synoviolin Antibody (4H4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Synoviolin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Synoviolin antibody (4H4) is available as the non-conjugated format. Synoviolin, also known as SYVN1 or HRD1, is a 617 amino acid multi-pass membrane protein that plays a crucial role in the endoplasmic reticulum-associated degradation (ERAD) pathway, which maintains cellular homeostasis by targeting misfolded or damaged proteins for degradation. Synoviolin (4H4) antibody recognizes a protein predominantly localized in the endoplasmic reticulum, where Synoviolin functions as an E3 ubiquitin-protein ligase, facilitating the ubiquitination process that marks proteins for destruction. Synoviolin′s location in the endoplasmic reticulum is integral to the quality control system, ensuring that only properly folded proteins proceed to functional destinations. Dysregulation of Synoviolin has been implicated in various diseases, including rheumatoid arthritis, where upregulation suggests a potential role in disease pathogenesis. Synoviolin monoclonal antibody (4H4) enables researchers to gain valuable insights into protein degradation mechanisms and Synoviolin′s implications in disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Synoviolin Antibody (4H4) References:

1. Human HRD1 is an E3 ubiquitin ligase involved in degradation of proteins from the endoplasmic reticulum.  |  Kikkert, M., et al. 2004. J Biol Chem. 279: 3525-34. PMID: 14593114
2. Multiprotein complexes that link dislocation, ubiquitination, and extraction of misfolded proteins from the endoplasmic reticulum membrane.  |  Lilley, BN. and Ploegh, HL. 2005. Proc Natl Acad Sci U S A. 102: 14296-301. PMID: 16186509
3. Resistance to endoplasmic reticulum stress is an acquired cellular characteristic of rheumatoid synovial cells.  |  Yamasaki, S., et al. 2006. Int J Mol Med. 18: 113-7. PMID: 16786162
4. The roles of synoviolin in crosstalk between endoplasmic reticulum stress-induced apoptosis and p53 pathway.  |  Yamasaki, S., et al. 2007. Cell Cycle. 6: 1319-23. PMID: 17582219
5. Synoviolin, protein folding and the maintenance of joint homeostasis.  |  Yagishita, N., et al. 2008. Nat Clin Pract Rheumatol. 4: 91-7. PMID: 18235538
6. SEL1L and HRD1 are involved in the degradation of unassembled secretory Ig-mu chains.  |  Cattaneo, M., et al. 2008. J Cell Physiol. 215: 794-802. PMID: 18314878
7. Novel functions of ubiquitin ligase HRD1 with transmembrane and proline-rich domains.  |  Omura, T., et al. 2008. J Pharmacol Sci. 106: 512-9. PMID: 18344614
8. Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP.  |  Hosokawa, N., et al. 2008. J Biol Chem. 283: 20914-24. PMID: 18502753
9. Activation of synoviolin promoter in rheumatoid synovial cells by a novel transcription complex of interleukin enhancer binding factor 3 and GA binding protein alpha.  |  Izumi, T., et al. 2009. Arthritis Rheum. 60: 63-72. PMID: 19116932
10. Synoviolin is not a pathogenic factor for auto-inflammatory diseases.  |  Matsumoto, T., et al. 2021. Biochem Biophys Res Commun. 558: 183-188. PMID: 33932778
11. Synoviolin inhibits the inflammatory cytokine secretion of Müller cells by reducing NLRP3.  |  Zhang, J., et al. 2022. J Mol Endocrinol. 68: 125-136. PMID: 34874278
12. Synoviolin alleviates GSDMD-mediated periodontitis by suppressing its stability.  |  Pang, Y., et al. 2023. Immun Inflamm Dis. 11: e880. PMID: 37506160

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Synoviolin Antibody (4H4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Synoviolin Antibody (4H4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Synoviolin Antibody (4H4) Specifications

Alternate Product Name: Synoviolin

Product Clone Name: 4H4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 238-318

Epitope Target: Synoviolin

Epitope Species: human

Product Reactivity/Detection: Synoviolin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 50 µg IgG_2b kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Synoviolin Antibody (4H4): N/A